Recombinant Human APAF1 protein, His-B2M-tagged

• Cat.No.: APAF1-018H
• Product Overview: Recombinant Human APAF1 protein(O14727)(104-415aa), fused with N-terminal 6xHis tag and B2M tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His-B2M
• Form: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Molecular Mass: 52.4 kDa
• Protein length: 104-415aa
• AA Sequence: SGITSYVRTVLCEGGVPQRPVVFVT RKKLVNAIQQKLSKLKGEPGWVTIH GMAGCGKSVLAAEAVRDHSLLEGCF PGGVHWVSVGKQDKSGLLMKLQNLC TRLDQDESFSQRLPLNIEEAKDRLR ILMLRKHPRSLLILDDVWDSWVLKA FDSQCQILLTTRDKSVTDSVMGPKY VVPVESSLGKEKGLEILSLFVNMKK ADLPEQAHSIIKECKGSPLVVSLIG ALLRDFPNRWEYYLKQLQNKQFKRI RKSSSYDYEALDEAMSISVEMLRED IKDYYTDLSILQKDVKVPTKVLCIL WDMETEEVEDIL
• Purity: Greater than 85% as determined by SDS-PAGE.
• Storage: Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Gene Information

• Gene Name: APAF1 apoptotic peptidase activating factor 1 [ Homo sapiens ]
• Official Symbol: APAF1
• Synonyms: APAF1; apoptotic peptidase activating factor 1; apoptotic peptidase activating factor , apoptotic protease activating factor; apoptotic protease-activating factor 1; APAF 1; CED4; APAF-1; DKFZp781B1145
• Gene ID: 317
• mRNA Refseq: NM_001160
• Protein Refseq: NP_001151
• MIM: 602233
• UniProt ID: O14727