"APOL1" Related Products

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Recombinant Human APOL1 Protein, His-tagged

Cat.No.: APOL1-582H
Product Overview: Recombinant human APOL1 (Met 1-Leu398) (Q2KHQ6) was expressed, with a C-terminal polyhistidine tag.
Source: Baculovirus-Insect Cells
Species: Human
Tag: His
Predicted N Terminal: Glu 28
Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly, 3mM DTT, 5%~8% trehalose and mannitol.
Molecular Mass: The secreted recombinant human APOL1 consists of 381 amino acids and predicts a molecular mass of 42.5 KDa. The apparent molecular mass of the protein is approximately 44 KDa in SDS-PAGE under reducing conditions due to glycosylation.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >91 % as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: APOL1 apolipoprotein L, 1 [ Homo sapiens ]
Official Symbol: APOL1
Synonyms: APOL1; apolipoprotein L, 1; APOL; apolipoprotein L1; APO-L; FSGS4; APOL-I;
Gene ID: 8542
mRNA Refseq: NM_001136540
Protein Refseq: NP_001130012
MIM: 603743
UniProt ID: O14791
Chromosome Location: 22q13.1
Pathway: African trypanosomiasis, organism-specific biosystem; African trypanosomiasis, conserved biosystem;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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