Recombinant Human Arginase, Type II, His-tagged

• Cat.No.: ARG2-2576H
• Product Overview: Recombinant human ARG2, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography. MW =38.3kDa(353aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Arginase is a manganese-containing enzyme which catalyzes the hydrolysis of arginine to ornithine and urea. It is the final enzyme of the urea cycle. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic cross-reactivity and physiologic function. The type II isoform is located in the mitochondria and expressed in extra-hepatic tissues, especially kidney. It is thought to play a role in nitric oxide and polyamine metabolism.
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MVHSVAVIGA PFSQGQKRKG VEHGPAAIRE AGLMKRLSSL GCHLKDFGDL SFTPVPKDDL YNNLIVNPRS VGLANQELAE VVSRAVSDGY SCVTLGGDHS LAIGTISGHA RHCPDLCVVW VDAHADINTP LTTSSGNLHG QPVSFLLREL QDKVPQLPGF SWIKPCISSA SIVYIGLRDV DPPEHFILKN YDIQYFSMRD IDRLGIQKVM ERTFDLLIGK RQRPIHLSFD IDAFDPTLAP ATFTPVVGGL TYREGMYIAE EIHNTGLLSA LDLVEVNPQL ATSEEEAKTT ANLAVDVIAS SFGQTREGGH IVYDQLPTPS SPDESENQAR VRI
• Purity: > 95% by SDS – PAGE.
• Form: Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 10% Glycerol.
• Concentration: 0.5 mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: ARG2 arginase, type II [ Homo sapiens ]
• Synonyms: ARG2; arginase, type II; arginase-2, mitochondrial; arginase 2; kidney arginase; type II arginase; nonhepatic arginase; kidney-type arginase; non-hepatic arginase; L-arginine ureahydrolase; L-arginine amidinohydrolase; EC 3.5.3.1
• Gene ID: 384
• mRNA Refseq: NM_001172
• Protein Refseq: NP_001163
• MIM: 107830
• UniProt ID: P78540
• Chromosome Location: 14q24.1-q24.3
• Pathway: Arginine and proline metabolism; Metabolic pathways
• Function: arginase activity; hydrolase activity; metal ion binding; nitric-oxide synthase binding; protein binding

Citation

Ranolazine may exert its beneficial effects by increasing myocardial adenosine levels

Journal: American Journal of Physiology - Heart and Circulatory Physiology    PubMed ID: 31834840    Data: 2021/1/1

Authors: D. Elizabeth Le, Catherine M. Davis, Sanjiv Kaul

Article Snippet:The ability of ranolazine to inhibit cN-II and CD73 hydrolysis of the 5′-phosphate group from its substrate, 5′-AMP, was measured using the Malachite Green Phosphate Detection Kit (R&D Systems, Minneapolis, MN).The ability of ranolazine to inhibit cN-II and CD73 hydrolysis of the 5′-phosphate group from its substrate, 5′-AMP, was measured using the Malachite Green Phosphate Detection Kit (R&D Systems, Minneapolis, MN).. Recombinant human cN-II, His-tagged (Creative BioMart, Shirley, NY), and CD73 (R&D Systems, Minneapolis, MN), 0.001 μg, were incubated with 35.7 μM 5′-AMP in the presence of 10 μM ranolazine or vehicle for 20 min at 37°C before addition of Malachite green reagents.. Microwell plates were read on a Victor3 plate reader (Perkin Elmer, Waltham, MA) at 620 nm.Microwell plates were read on a Victor3 plate reader (Perkin Elmer, Waltham, MA) at 620 nm.