Recombinant Human ASL protein, His&GST-tagged

• Cat.No.: ASL-0251H
• Product Overview: Recombinant Human ASL protein(P04424)(Met1-Ala464), fused with His and GST tag, was expressed in Insect Cells.

Specification

• Source: Insect Cells
• Species: Human
• Tag: His&GST
• Form: Lyophilized from sterile 20mM Tris,500mM Nacl,3mM DTT, pH 8.0,10% glycerol.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Molecular Mass: The recombinant human ASL/GST chimera consists of 701 amino acids and has a calculated molecular mass of 79.5 kDa. The recombinant protein migrates approximately 68 kDa band in SDS-PAGE under reducing conditions.
• Proteinlength: Met1-Ala464
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: ASL argininosuccinate lyase [ Homo sapiens ]
• Official Symbol: ASL
• Synonyms: ASL; argininosuccinate lyase; arginosuccinase; argininosuccinase; ASAL
• Gene ID: 435
• mRNA Refseq: NM_000048
• Protein Refseq: NP_000039
• MIM: 608310
• UniProt ID: P04424