Recombinant Human ASS1, His-tagged

• Cat.No.: ASS1-30H
• Product Overview: Recombinant Human ASS1 (Met1-Lys412) fused with a 6His tag at the N-terminus, was expressed in E. coli.

Specification

• Cat. No.: ASS1-30H
• Description: The protein encoded by this gene catalyzes the penultimate step of the arginine biosynthetic pathway. There are approximately 10 to 14 copies of this gene including the pseudogenes scattered across the human genome, among which the one located on chromosome 9 appears to be the only functional gene for argininosuccinate synthetase. Mutations in the chromosome 9 copy of this gene cause citrullinemia. Two transcript variants encoding the same protein have been found for this gene.
• Source: E. coli
• Formulation: Supplied as a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, 50mM Imidazole, 1mM DTT, 40% Glycerol, pH 7.5
• Purity: Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
• Shipping: The product is shipped on dry ice/ice packs.
• Endotoxin: Less than 0.1 ng/µg (1 IEU/µg).
• Storage: Store at < -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.
• OfficialSymbol: ASS1

Gene Information

• Gene Name: ASS1 argininosuccinate synthase 1 [ Homo sapiens ]
• Synonyms: ASS1; argininosuccinate synthase 1; ASS; CTLN1; argininosuccinate synthase; OTTHUMP00000022363; OTTHUMP00000022364; citrulline-aspartate ligase; citrulline--aspartate ligase; argininosuccinate synthetase 1; EC 6.3.4.5; argininosuccinate synthetase
• Gene ID: 445
• mRNA Refseq: NM_000050
• Protein Refseq: NP_000041
• MIM: 603470
• UniProt ID: P00966
• Chromosome Location: 9q34.1
• Pathway: Alanine, aspartate and glutamate metabolism; Arginine and proline metabolism; Metabolism of amino acids and derivatives
• Function: ATP binding; argininosuccinate synthase activity; protein binding; toxin binding