Recombinant Human ASS1 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: ASS1-2317H
• Product Overview: ASS1 MS Standard C13 and N15-labeled recombinant protein (NP_446464) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The protein encoded by this gene catalyzes the penultimate step of the arginine biosynthetic pathway. There are approximately 10 to 14 copies of this gene including the pseudogenes scattered across the human genome, among which the one located on chromosome 9 appears to be the only functional gene for argininosuccinate synthetase. Mutations in the chromosome 9 copy of this gene cause citrullinemia. Two transcript variants encoding the same protein have been found for this gene.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 46.5 kDa
• AA Sequence: MSSKGSVVLAYSGGLDTSCILVWLK EQGYDVIAYLANIGQKEDFEEARKK ALKLGAKKVFIEDVSREFVEEFIWP AIQSSALYEDRYLLGTSLARPCIAR KQVEIAQREGAKYVSHGATGKGNDQ VRFELSCYSLAPQIKVIAPWRMPEF YNRFKGRNDLMEYAKQHGIPIPVTP KNPWSMDENLMHISYEAGILENPKN QAPPGLYTKTQDPAKAPNTPDILEI EFKKGVPVKVTNVKDGTTHQTSLEL FMYLNEVAGKHGVGRIDIVENRFIG MKSRGIYETPAGTILYHAHLDIEAF TMDREVRKIKQGLGLKFAELVYTGF WHSPECEFVRHCIAKSQERVEGKVQ VSVLKGQVYILGRESPLSLYNEELV SMNVQGDYEPTDATGFININSLRLK EYHRLQSKVTAKTRTRPLEQKLISE EDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: ASS1 argininosuccinate synthase 1 [ Homo sapiens (human) ]
• Official Symbol: ASS1
• Synonyms: ASS1; argininosuccinate synthase 1; argininosuccinate synthetase, ASS; argininosuccinate synthase; CTLN1; citrulline-aspartate ligase; citrulline--aspartate ligase; argininosuccinate synthetase 1; ASS
• Gene ID: 445
• mRNA Refseq: NM_054012
• Protein Refseq: NP_446464
• MIM: 603470
• UniProt ID: P00966