Recombinant Human ASTL Protein (24-431 aa), His-Myc-tagged ASTL-2574H

Recombinant Human ASTL Protein (24-431 aa), His-Myc-tagged

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Recombinant Human ASTL Protein (24-431 aa), His-Myc-tagged

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Cat.No. : ASTL-2574H
Product Overview : Recombinant Human ASTL Protein (24-431 aa) is produced by E. coli expression system. This protein is fused with a 10xHis tag at the N-terminal and a Myc tag at the C-terminal. Research Area: Signal Transduction. Protein Description: Full Length of Mature Protein.
Description : Oocyte-specific oolemmal receptor involved in sperm and egg adhesion and fertilization. Plays a role in the polyspermy inhibition. Probably acts as a protease for the post-fertilization cleavage of ZP2. Cleaves the sperm-binding ZP2 at the surface of the zona pellucida after fertilization and cortical granule exocytosis, rendering the zona pellucida unable to support further sperm binding.
Source : E. coli
Species : Human
Tag : His-Myc
Form : Tris-based buffer,50% glycerol
Molecular Mass : 50.6 kDa
Protein length : 24-431 aa
AA Sequence : APLASSCAGACGTSF PDGLTPEGTQASGDK DIPAINQGLILEETP ESSFLIEGDIIRPSP FRLLSATSNKWPMGG SGVVEVPFLLSSKYD EPSRQVILEALAEFE RSTCIRFVTYQDQRD FISIIPMYGCFSSVG RSGGMQVVSLAPTCL QKGRGIVLHELMHVL GFWHEHTRADRDRYI RVNWNEILPGFEINF IKSQSSNMLTPYDYS SVMHYGRLAFSRRGL PTITPLWAPSVHIGQ RWNLSASDITRVLKL YGCSPSGPRPRGRGS HAHSTGRSPAPASLS LQRLLEALSAESRSP DPSGSSAGGQPVPAG PGESPHGWESPALKK LSAEASARQPQTLAS SPRSRPGAGAPGVAQ EQSWLAGVSTKPTVP SSEAGIQPVPVQGSP ALPGGCVPRNHFKGM SED
Purity : > 85% as determined by SDS-PAGE.
Notes : Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
Storage : The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
Concentration : A hardcopy of COA will be sent along with the products. Please refer to it for detailed information.
Figure :

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Gene Name : ASTL astacin-like metallo-endopeptidase (M12 family) [ Homo sapiens ]
Official Symbol : ASTL
Synonyms : ASTL; astacin-like metallo-endopeptidase (M12 family); ovastacin; oocyte astacin; MGC129843;
Gene ID : 431705
mRNA Refseq : NM_001002036
Protein Refseq : NP_001002036
MIM : 608860
UniProt ID : Q6HA08

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