Recombinant Human ATG13, GST-tagged

• Cat.No.: ATG13-6972H
• Product Overview: Recombinant full-length human ATG13 was expressed by baculovirus in E.coli using an N-terminal GST tag.

Specification

• Description: ATG13 is a protein that is part of the autophagy complex which is the major route by which cytoplasmic contents are delivered to the lysosome for degradation. ATG13 localizes on the autophagic isolation membrane and is essential for autophagosome formation. In mammals, ATG13 interacts with ATG1 (ULK1/2) and FIP200 to form the autophagy. ATG101 can also bind to ATG13 protein and is important for the stability and basal phosphorylation of ATG13 and ULK1. mTOR can suppress the autophagy process through direct regulation of the ULK1-Atg13-FIP200 complex where mTOR has been shown tophosphorylate ATG13.
• Source: E. coli
• Species: Human
• Tag: GST
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Molecular Mass: ~96 kDa
• Protein length: Full length
• Purity: >70%
• Applications: Western Blot
• Storage: Store at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. Avoid freeze/thaw cycles.
• Concentration: 0.1 μg/μ

Gene Information

• Gene Name: ATG13 ATG13 autophagy related 13 homolog (S. cerevisiae) [ Homo sapiens ]
• Official Symbol: ATG13
• Synonyms: ATG13; ATG13 autophagy related 13 homolog (S. cerevisiae); KIAA0652; autophagy-related protein 13; FLJ20698
• Gene ID: 9776
• mRNA Refseq: NM_001142673
• Protein Refseq: NP_001136145
• UniProt ID: O75143
• Chromosome Location: 11p11.2
• Pathway: mTOR signaling pathway, organism-specific biosystem
• Function: protein binding; protein kinase binding