Recombinant Human ATP5O, 24-213 aa, His-tagged

• Cat.No.: ATP5O-950H
• Product Overview: Recombinant human ATP5O protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. MW = 23.1 kDa (211aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: ATP synthase subunit O, also known as ATP5O, localizes to the mitochondria and catalyzes ATP synthesis. The protein is a component of the F-type ATPase found in the mitochondrial matrix. F-type ATPases are composed of a catalytic core and a membrane proton channel. The encoded protein appears to be part of the connector linking these two components and may be involved in transmission of conformational changes or proton conductance.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 40% glycerol, 0.2M NaCl.
• Purity: > 95% by SDS - PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MFAKLVRPPV QVYGIEGRYA TALYSAASKQ NKLEQVEKEL LRVAQILKEP KVAASVLNPY VKRSIKVKSL NDITAKERFS PLTTNLINLL AENGRLSNTQ GVVSAFSTMM SVHRGEVPCT VTSASPLEEA TLSELKTVLK SFLSQGQVLK LEAKTDPSIL GGMIVRIGEK YVDMSVKTKI QKLGRAMREI V.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: Alzheimer"s disease; Huntington"s disease; Metabolic pathways; Oxidative phosphorylation; Parkinson"s disease; Respiratory electron transport, ATP synthesis by chemiosmotic coupling, and heat production by uncoupling proteins

Gene Information

• Gene Name: ATP5O ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit [ Homo sapiens ]
• Synonyms: ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit; ATPO; OSCP; ATP5O;ATP synthase subunit O, mitochondrial; human ATP synthase OSCP subunit; oligomycin sensitivity conferral protein; oligomycin sensitivity conferring protein; Oligomycin sensitivity conferral protein; human ATP synthase OSCP subunit, oligomycin sensitivity conferring protein9
• Gene ID: 539
• mRNA Refseq: NM_001697
• Protein Refseq: NP_001688
• MIM: 600828
• UniProt ID: P48047
• Chromosome Location: 21q22.1-q22.2; 21q22.11
• Function: contributes_to ATPase activity; drug binding; hydrogen ion transporting ATP synthase activity, rotational mechanism; transmembrane transporter activity; transporter activity