| Description : |
This gene encodes bifunctional mitochondrial protein that has both RNA-binding and hydratase activities. The encoded protein is a methylglutaconyl-CoA hydratase that catalyzes the hydration of 3-methylglutaconyl-CoA to 3-hydroxy-3-methyl-glutaryl-CoA, a critical step in the leucine degradation pathway. This protein also binds AU-rich elements (AREs) found in the 3' UTRs of rapidly decaying mRNAs including c-fos, c-myc and granulocyte/ macrophage colony stimulating factor. ARE elements are involved in directing RNA to rapid degradation and deadenylation. This protein is localizes to the mitochondrial matrix and the inner mitochondrial membrane and may be involved in mitochondrial protein synthesis. Mutations in this gene are the cause of 3-methylglutaconic aciduria, type I. Alternative splicing results in multiple transcript variants. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
35.61 kDa |
| AA Sequence : |
MAAAVAAAPGALGSLHAGGARLVAA CSAWLCPGLRLPGSLAGRRAGPAIW AQGWVPAAGGPAPKRGYSSEMKTED ELRVRHLEEENRGIVVLGINRAYGK NSLSKNLIKMLSKAVDALKSDKKVR TIIIRSEVPGIFCAGADLKERAKMS SSEVGPFVSKIRAVINDIANLPVPT IAAIDGLALGGGLELALACDIRVAA SSAKMGLVETKLAIIPGGGGTQRLP RAIGMSLAKELIFSARVLDGKEAKA VGLISHVLEQNQEGDAAYRKALDLA REFLPQGPVAMRVAKLAINQGMEVD LVTGLAIEEACYAQTIPTKDRLEGL LAFKEKRPPRYKGETRTRPLEQKLI SEEDLAANDILDYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
