|Product Overview:||Recombinant Human BAI2(Phe21-Asp911) fused with Fc region of Human IgG1 at C-terminal was expressed in HEK293.|
|Description:||Brain angiogenesis inhibitor 2 (BAI2), along with BAI1 and BAI3, form a subfamily of brain specific adhesion GPCRs. They are large, glycosylated molecules, approximately 180-220 kDa, and play a role in neuronal synapse formation and maintenance. Mature human BAI2 consists of a 903 amino acid (aa) N-terminal extracellular domain (ECD) containing four TSP1 domains, a hormone binding domain (HBD), a GAIN domain and a GPS protease sensitive linker, followed by a region with seven transmembrane segments and a 410 aa C-terminal cytoplasmic domain. Within the N-terminal ECD, human BAI2 shares 96% aa sequence identity with both mouse and rat BAI2. BAI2 is expressed primarily in neurons and astrocytes of the hippocampus and cerebral cortex in the brain. To become functional, BAI2 might require activation by cleavage at the GPS domain. BAI family members are known to bind the C1q-like complement (C1qL) family via thrombospondin type 1 repeats (TSRs) which are conserved between BAI members. BAI2 has been shown to suppress expression of VEGF leading to increased neurogenesis in the dentate gyrus of the hippocampus.|
|Predicted N Terminal:||Phe21|
|Form:||Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.|
|Bio-activity:||Measured by its binding ability in a functional ELISA. When Recombinant Human C1qL3 is immobilized at 2 μg/mL, 100 μL/well, the concentration of Recombinant Human BAI2 Fc Chimera that produces 50% of the optimal binding response is approximately 25-150 ng/mL.|
|Molecular Mass:||Predicted Molecular Mass: 123 kDa
SDS-PAGE: 128-200 kDa, reducing conditions
|Endotoxin:||<0.1 EU per 1 μg of the protein by the LAL method.|
|Purity:||>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.|
|Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
|Reconstitution:||Reconstitute at 250 μg/mL in PBS.|
|Gene Name:||BAI2 brain-specific angiogenesis inhibitor 2 [ Homo sapiens ]|
|Synonyms:||BAI2; brain-specific angiogenesis inhibitor 2; Brain-specific angiongenesis inhibitor-2;|
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