Recombinant Human BCAT1, GST-tagged

• Cat.No.: BCAT1-10169H
• Product Overview: Recombinant Human BCAT1 protein, fused to GST-tag, was expressed in E.coli and purified by GSH-sepharose.

Specification

• Species: Human
• Source: E.coli
• Tag: GST
• Protein Length: 1-320a.a.
• Description: This gene encodes the cytosolic form of the enzyme branched-chain amino acid transaminase. This enzyme catalyzes the reversible transamination of branched-chain alpha-keto acids to branched-chain L-amino acids essential for cell growth. Two different clinical disorders have been attributed to a defect of branched-chain amino acid transamination: hypervalinemia and hyperleucine-isoleucinemia. As there is also a gene encoding a mitochondrial form of this enzyme, mutations in either gene may contribute to these disorders. Alternatively spliced transcript variants have been described.
• Storage: The protein is stored in PBS buffer at -20℃. Avoid repeated freezing and thawing cycles.
• Storage Buffer: 1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 100mM GSH and 1% Triton X-100,15%glycerol.

Gene Information

• Gene Name: BCAT1 branched chain amino-acid transaminase 1, cytosolic [ Homo sapiens ]
• Official Symbol: BCAT1
• Synonyms: BCAT1; branched chain amino-acid transaminase 1, cytosolic; BCT1, branched chain aminotransferase 1, cytosolic; branched-chain-amino-acid aminotransferase, cytosolic; placental protein 18; branched chain aminotransferase 1, cytosolic; BCT1; PP18; BCATC; ECA39; MECA39; PNAS121; DKFZp686E12175
• Gene ID: 586
• mRNA Refseq: NM_001178091
• Protein Refseq: NP_001171562
• MIM: 113520
• UniProt ID: P54687
• Chromosome Location: 12pter-q12
• Pathway: Branched-chain amino acid catabolism, organism-specific biosystem; Leucine degradation, leucine => acetoacetate + acetyl-CoA, organism-specific biosystem; Leucine degradation, leucine => acetoacetate + acetyl-CoA, conserved biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem
• Function: L-isoleucine transaminase activity; L-leucine transaminase activity; L-valine transaminase activity; branched-chain-amino-acid transaminase activity; transferase activity