Recombinant Human BCDIN3D Protein, Myc/DDK-tagged, C13 and N15-labeled

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Recombinant Human BCDIN3D Protein, Myc/DDK-tagged, C13 and N15-labeled

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Cat.No. : BCDIN3D-3359H
Product Overview : BCDIN3D MS Standard C13 and N15-labeled recombinant protein (NP_859059) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.
Description : This gene encodes an RNA methyltransferase which belongs to the rossmann fold methyltransferase family, and serves as a 5'-methylphosphate capping enzyme that is specific for cytoplasmic histidyl tRNA. The encoded protein contains an S-adenosylmethionine binding domain and uses the methyl group donor, S-adenosylmethionine. This gene is overexpressed in breast cancer cells, and is related to the tumorigenic phenotype and poor prognosis of breast cancer. The encoded protein is thought to promote the cellular invasion of breast cancer cells, by downregulating the expression of tumor suppressor miRNAs through the dimethylation of the 5-monophosphate of the corresponding precursor miRNAs.
Source : HEK293
Species : Human
Tag : Myc/DDK
Molecular Mass : 33.2 kDa
AA Sequence : MAVPTELDGG SVKETAAEEE SRVLAPGAAP FGNFPHYSRF HPPEQRLRLL PPELLRQLFP ESPENGPILG LDVGCNSGDL SVALYKHFLS LPDGETCSDA SREFRLLCCD IDPVLVKRAE KECPFPDALT FITLDFMNQR TRKVLLSSFL SQFGRSVFDI GFCMSITMWI HLNHGDHGLW EFLAHLSSLC HYLLVEPQPW KCYRAAARRL RKLGLHDFDH FHSLAIRGDM PNQIVQILTQ DHGMELICCF GNTSWDRSLL LFRAKQTIET HPIPESLIEK GKEKNRLSFQ KQTRTRPLEQ KLISEEDLAA NDILDYKDDD DKV
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 3 months from receipt of products under proper storage and handling conditions.
Storage : Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
Concentration : 50 μg/mL as determined by BCA
Storage Buffer : 100 mM glycine, 25 mM Tris-HCl, pH 7.3.
SDS-PAGE :

Gene Name : BCDIN3D BCDIN3 domain containing [ Homo sapiens (human) ]
Official Symbol : BCDIN3D
Synonyms : BCDIN3D; BCDIN3 domain containing; RNA 5'-monophosphate methyltransferase; BCDIN3 domain containing RNA methyltransfease; BCDIN3 domain-containing protein; pre-miRNA 5'-monophosphate methyltransferase; probable methyltransferase BCDIN3D; EC 2.1.1.-
Gene ID : 144233
mRNA Refseq : NM_181708
Protein Refseq : NP_859059
UniProt ID : Q7Z5W3

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