Recombinant Mouse Bcdin3d Protein, Myc/DDK-tagged

• Cat.No.: Bcdin3d-1846M
• Product Overview: Purified recombinant protein of mouse full-length BCDIN3 domain containing (Bcdin3d), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: O-methyltransferase that specifically monomethylates 5'-monophosphate of cytoplasmic histidyl tRNA (tRNA(His)), acting as a capping enzyme by protecting tRNA(His) from cleavage by DICER1. Also able, with less efficiently, to methylate the 5' monophosphate of a subset of pre-miRNAs, acting as a negative regulator of miRNA processing. The 5' monophosphate of pre-miRNAs is recognized by DICER1 and is required for pre-miRNAs processing: methylation at this position reduces the processing of pre-miRNAs by DICER1. Was also reported to mediate dimethylation of pre-miR-145; however dimethylation cannot be reproduced by another group which observes a monomethylation of pre-miR-145.
• Molecular Mass: 32 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Bcdin3d BCDIN3 domain containing [ Mus musculus (house mouse) ]
• Official Symbol: Bcdin3d
• Synonyms: Bcdin3d; BCDIN3 domain containing; AV138748; 4930556P03Rik; RNA 5'-monophosphate methyltransferase; BCDIN3 domain-containing protein; pre-miRNA 5'-monophosphate methyltransferase; probable methyltransferase BCDIN3D; EC 2.1.1.-
• Gene ID: 75284
• mRNA Refseq: NM_029236
• Protein Refseq: NP_083512
• UniProt ID: Q91YP1