Recombinant Human BLNK Protein, His-tagged

• Cat.No.: BLNK-406H
• Product Overview: Recombinant Human BLNK, transcript variant 1, fused with C-terminal His was expressed in E. coli.

Specification

• Description: This gene encodes a cytoplasmic linker or adaptor protein that plays a critical role in B cell development. This protein bridges B cell receptor-associated kinase activation with downstream signaling pathways, thereby affecting various biological functions. The phosphorylation of five tyrosine residues is necessary for this protein to nucleate distinct signaling effectors following B cell receptor activation. Mutations in this gene cause hypoglobulinemia and absent B cells, a disease in which the pro- to pre-B-cell transition is developmentally blocked. Deficiency in this protein has also been shown in some cases of pre-B acute lymphoblastic leukemia. Alternatively spliced transcript variants have been found for this gene.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Lyophilized from a 0.2 µM filtered solution of 20mM PB, 150mM NaCl, 1mM DTT, pH 7.2
• Molecular Mass: 51.5kD
• AA Sequence: MDKLNKITVPASQKLRQLQKMVHDI KNNEGGIMNKIKKLKVKAPPSVPRR DYASESPADEEQQWSDDFDSDYENP DEHSDSEMYVMPAEENADDSYEPPP VEQETRPVHPALPFARGEYIDNRSS QRHSPPFSKTLPSKPSWPSEKARLT STLPALTALQKPQVPPKPKGLLEDE ADYVVPVEDNDENYIHPTESSSPPP EKAPMVNRSTKPNSSTPASPPGTAS GRNSGAWETKSPPPAAPSPLPRAGK KPTTP
• Endotoxin: Endotoxin level is <0.1 ng/µg of protein (<1EU/µg).
• Purity: >95% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 µg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Gene Information

• Gene Name: BLNK B-cell linker [ Homo sapiens ]
• Official Symbol: BLNK
• Synonyms: BLNK; B-cell linker; B-cell linker protein; B cell adaptor containing SH2 domain; B cell activation; B cell adapter containing a SH2 domain protein; BASH; bca; BLNK s; Ly57; SLP 65; SLP65; Src homology [SH2] domain containing leukocyte protein of 65 kD; B-cell activation; B cell linker protein; cytoplasmic adapter protein; B-cell adapter containing a SH2 domain protein; B-cell adapter containing a Src homology 2 domain protein; Src homology 2 domain-containing leukocyte protein of 65 kDa; Src homology [SH2] domain-containing leukocyte protein of 65 kD; AGM4; LY57; BLNK-S; SLP-65; MGC111051
• Gene ID: 29760
• mRNA Refseq: NM_013314
• Protein Refseq: NP_037446
• MIM: 604515
• UniProt ID: Q8WV28