Recombinant Human BTN1A1 protein, Fc-tagged

• Cat.No.: BTN1A1-333H
• Product Overview: Recombinant Human BTN1A1 protein (Ala27-Arg242), fused to human IgG1 Fc tag at C-terminus, was expressed in human 293 cells (HEK293).

Specification

• Description: Butyrophilin subfamily 1 member A1 (BTN1A1) is also known as BTN, which is a member of the immunoglobulin superfamily and the major protein associated with fat droplets in the milk. BTN1A1 may have a cell surface receptor function. The human butyrophilin gene is localized in the major histocompatibility complex (MHC) class I region of 6p and may have arisen relatively recently in evolution by the shuffling of exons between 2 ancestral gene families. Furthermore, BTN1A1 regulates the amount of lipids and size of droplets expressed in milk and inhibits the proliferation of CD4 and CD8 T-cells activated by anti-CD3 antibodies, T-cell metabolism and IL2 and IFNG secretion.
• Source: HEK293
• Species: Human
• Tag: human IgG1 Fc
• Form: Lyophilized from 0.22 um filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 50.6 kDa. As a result of glycosylation, the protein migrates as 55-66 kDa under reducing (R) condition, and 116-130 kDa under non-reducing (NR) condition (SDS-PAGE).
• Protein length: 216
• Endotoxin: Less than 1.0 EU per ug by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 centigrade to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: BTN1A1
• Official Symbol: BTN1A1
• Synonyms: BTN1A1; butyrophilin, subfamily 1, member A1; BTN; butyrophilin subfamily 1 member A1; BT; bK14H9.2 (butyrophilin, subfamily 1, member A1)
• Gene ID: 696
• mRNA Refseq: NM_001732
• Protein Refseq: NP_001723
• MIM: 601610
• UniProt ID: Q13410