Recombinant Human CAMK2B Protein, GST-Tagged CAMK2B-0328H

Recombinant Human CAMK2B Protein, GST-Tagged

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Recombinant Human CAMK2B Protein, GST-Tagged

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Cat.No. : CAMK2B-0328H
Product Overview : Human CAMK2B (NP_742078.1, 1 a.a. - 503 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells. cells.
Description : The product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a beta chain. It is possible that distinct isoforms of this chain have different cellular localizations and interact differently with calmodulin. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]
Source : Insect Cell
Species : Human
Tag : GST
Form : Liquid
Bio-activity : The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate, Mg(or Mn)/ATP, and Ca/Calmodulin. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip TM3000. Substrate: GS peptide. ATP: 100 μM.
Molecular Mass : 83 kDa
Purity : 74 % by SDS-PAGE/CBB staining
Applications : Functional Study
SDS-PAGE
Storage : Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
Storage Buffer : In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)
Quality Control Testing :

Loading 1 μg protein in SDS-PAGE

Result of activity analysis :

Gene Name : CAMK2B calcium/calmodulin-dependent protein kinase II beta [ Homo sapiens ]
Official Symbol : CAMK2B
Synonyms : CAMK2B; calcium/calmodulin-dependent protein kinase II beta; calcium/calmodulin dependent protein kinase (CaM kinase) II beta, CAMKB; calcium/calmodulin-dependent protein kinase type II subunit beta; calcium/calmodulin dependent protein kinase type II beta chain; CaM kinase II beta subunit; CaM kinase II beta chain; CAM2; CAMK2; proline rich calmodulin dependent protein kinase; caMK-II subunit beta; CaM-kinase II beta chain; proline rich calmodulin-dependent protein kinase; CAMKB; MGC29528;
Gene ID : 816
mRNA Refseq : NM_001220
Protein Refseq : NP_001211
MIM : 607707
UniProt ID : Q13554

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