Recombinant Human CAPN5 Protein, His/GST-tagged

• Cat.No.: CAPN5-12H
• Product Overview: Recombinant Human CAPN5(Phe2~Asn344) fused with His/GST tag at N-terminal was expressed in E. coli.

Specification

• Description: Calpains are calcium-dependent cysteine proteases involved in signal transduction in a variety of cellular processes. A functional calpain protein consists of an invariant small subunit and 1 of a family of large subunits. CAPN5 is one of the large subunits. Unlike some of the calpains, CAPN5 and CAPN6 lack a calmodulin-like domain IV. Because of the significant similarity to Caenorhabditis elegans sex determination gene tra-3, CAPN5 is also called as HTRA3.
• Source: E. coli
• Species: Human
• Tag: His/GST
• Form: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Molecular Mass: 69.4kDa
• Protein length: Phe2~Asn344
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
  If bio-activity of the protein is needed, please check active protein.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: CAPN5 calpain 5 [ Homo sapiens ]
• Official Symbol: CAPN5
• Synonyms: CAPN5; calpain 5; calpain-5; HTRA3; nCL 3; new calpain 3; calpain htra-3; nCL-3; FLJ46245
• Gene ID: 726
• mRNA Refseq: NM_004055
• Protein Refseq: NP_004046
• MIM: 602537
• UniProt ID: O15484