Recombinant Human CAPNS1 and CAPN2 Protein, His tagged

• Cat.No.: CAPN2-01H

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: CAPNS1: 26-268 aa CAPN2: 1-700 aa
• Description: The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene.
• Molecular Mass: CAPNS1: 26 kDa CAPN2: 82 kDa
• AA Sequence: CAPNS1: MGNVLGGLISGAGGGGGGGGGGGGGGGGGGGGTAMRILGGVISAISEAAAQYNPEPPPPRTHYSNIEANESEEVRQFRRLFAQLAGDDMEVSATELMNILNKVVTRHPDLKTDGFGIDTCRSMVAVMDSDTTGKLGFEEFKYLWNNIKRWQAIYKQFDTDRSGTICSSELPGAFEAAGFHLNEHLYNMIIRRYSDESGNMDFDNFISCLVRLDAMFRAFKSLDKDGTGQIQVNIQEWLQLTMYS CAPN2: MAGIAAKLAKDREAAEGLGSHDRAIKYLNQDYEALRNECLEAGTLFQDPSFPAIPSALGFKELGPYSSKTRGIEWKRPTEICADPQFIIGGATRTDICQGALGDCWLLAAIASLTLNEEILARVVPLNQSFQENYAGIFHFQFWQYGEWVEVVVDDRLPTKDGELLFVHSAEGSEFWSALLEKAYAKINGCYEALSGGATTEGFEDFTGGIAEWYELKKPPPNLFKIIQKALQKGSLLGCSIDITSAADSEAITFQKLVKGHAYSVTGAEEVESNGSLQKLIRIRNPWGEVEWTGRWNDNCPSWNTIDPEERERLTRRHEDGEFWMSFSDFLRHYSRLEICNLTPDTLTSDTYKKWKLTKMDGNWRRGSTAGGCRNYPNTFWMNPQYLIKLEEEDEDEEDGESGCTFLVGLIQKHRRRQRKMGEDMHTIGFGIYEVPEELSGQTNIHLSKNFFLTNRARERSDTFINLREVLNRFKLPPGEYILVPSTFEPNKDGDFCIRVFSEKKADYQAVDDEIEANLEEFDISEDDIDDGFRRLFAQLAGEDAEISAFELQTILRRVLAKRQDIKSDGFSIETCKIMVDMLDSDGSGKLGLKEFYILWTKIQKYQKIYREIDVDRSGTMNSYEMRKALEEAGFKMPCQLHQVIVARFADDQLIIDFDNFVRCLVRLETLFKIFKQLDPENTGTIELDLISWLCFSVLGKLAAALEHHHHHH
• Bio-Activity: 18.5 U/mg protein. One unit is defined as the amount of enzyme that cleaves 1pmol of the caspase substrate N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin per 15min at 37 centigrade.
• Purity: > 90% by SDS-PAGE
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Sterile 50 mM Tris, 100 mM NaCl, 40% Glycerol, 5mM EDTA, 5mM 2-Mercaptoethanol, pH7.4
• Concentration: 0.2 mg/mL by BCA
• GeneID 2: 826

Gene Information

• Gene Name: CAPN2 calpain 2 [ Homo sapiens (human) ]
• Official Symbol: CAPN2
• Synonyms: CAPN2; calpain 2, (m/II) large subunit; calpain-2 catalytic subunit; CANPL2; CANPml; mCANP; CANP 2; M-calpain; calpain M-type; millimolar-calpain; calpain-2 large subunit; calpain 2, large subunit; calpain large polypeptide L2; calpain, large polypeptide L2; calpain 2, large [catalytic] subunit; calcium-activated neutral proteinase 2; CANP2; FLJ39928
• Gene ID: 824
• mRNA Refseq: NM_001146068
• Protein Refseq: NP_001139540
• MIM: 114230
• UniProt ID: P17655
• Gene Name 2: CAPNS1 calpain small subunit 1 [ Homo sapiens (human) ]
• Official Symbol 2: CAPNS1
• Synonyms 2: CAPNS1; calpain, small subunit 1; CAPN4; calpain small subunit 1; 30K; CANP; CANPS; CDPS; CANP small subunit; calpain regulatory subunit; calpain, small polypeptide; calpain 4, small subunit (30K); calcium-dependent protease, small subunit; calcium-dependent protease small subunit 1; calcium-activated neutral proteinase small subunit; CSS1; CALPAIN4
• mRNA Refseq 2: NM_001003962
• Protein Refseq 2: NP_001003962
• MIM 2: 114170
• UniProt ID 2: P04632
• Gene ID 2: 826