"CAPZA1" Related Products

Expand
Expand
Expand

Recombinant Human CAPZA1/CAPZB Protein, His-tagged

Cat.No.: CAPZA1&CAPZB-869H
Product Overview: Recombinant Human CAPZA1&CAPZB(non-muscle isoform) with the alpha-1 SU untagged and beta-2 SU His-tagged was produced in HEK293 cells.
Description: The subunits of the heterodimeric capping protein CapZ possess a molecular mass of 34kDa (alpha 1-subunit) and 32kDa (beta 2-subunit, His-tagged). CapZ binds with high affinity in a Ca++-independent manner to the barbed end of actin filaments (1:1000 M/M). Ca++-insensitive barbed end capping protein. Kd actin binding = >1nM (barbed end) .
Source: HEK293
Species: Human
Tag : His
Form: 150mM NaCl, 20mM Hepes pH 7.4, 1mM DTT and 5% sucrose, when reconstituted with 1.0ml ultrapure water to obtain a 1.0 mg/ml solution.
Bio-activity: Capping activity: F-actin containing 20% pyrene actin was incubated with CapZ and diluted below the critical concentration. Depolymerisation was followed by fluorometry and compared to an actin reference without CapZ.
Complete capping was obtained at a molar ratio of 1 : 1000 (CapZ : Actin). All proteins were used as supplied, i.e. without dialysis. G-Actin stock solutions were pre-spun 1h, 100.000xg, 4 centigrade.
Molecular Mass: 66kDa
Purity: >94% by scanning densitometry from Coomassie G-250 stained SDS-Gels.
Storage: CapZ is stored at –70 centigrade upon arrival will be stable in performance for at least 6 months from the date of purchase. The solubilized protein is kept on ice and should be stored at -20 centigrade in glycerol or CryoProtect (Cat.#: 6011-01). Avoid repeated freeze / thaw cycles.
Shipping: At ambient temperature. Upon delivery store at -70 centigrade.
Gene Name: CAPZA1 capping protein (actin filament) muscle Z-line, alpha 1 [ Homo sapiens ]
Official Symbol: CAPZA1
Synonyms: CAPZA1; capping protein (actin filament) muscle Z-line, alpha 1; F-actin-capping protein subunit alpha-1; Cap Z; capZ alpha-1; CAPZ; CAZ1; CAPPA1;
Gene ID: 829
mRNA Refseq: NM_006135
Protein Refseq: NP_006126
MIM: 601580
UniProt ID: P52907

Online Inquiry

Note: There will be extra charge for optional service!
Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information


Price does not include shipping and packaging costs.Request quote for final price.

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.