Recombinant Human Carboxymethylenebutenolidase Homolog (Pseudomonas), His-tagged

• Cat.No.: CMBL-6960H
• Product Overview: Recombinant human CMBL protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-245aa
• Description: CMBL is a cysteine hydrolase of the dienelactone hydrolase family that is highly expressed in liver cytosol. CMBL is the human homolog of Pseudomonas dienelactone hydrolase, a protein that participates in the bacterial halocatechol degradation pathway. CMBL is inhibited by p-chloromercuribenzoate (PCMB) and is encoded by a gene that maps to human chromosome 5p15.2.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol, 1mM EDTA.
• Molecular Mass: 30.6 kDa (269aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMANEAY PCPCDIGHRL EYGGLGREVQ VEHIKAYVTK SPVDAGKAVI VIQDIFGWQL PNTRYIADMI SGNGYTTIVP DFFVGQEPWD PSGDWSIFPE WLKTRNAQKI DREISAILKY LKQQCHAQKI GIVGFCWGGT AVHHLMMKYS EFRAGVSVYG IVKDSEDIYN LKNPTLFIFA ENDVVIPLKD VSLLTQKLKE HCKVEYQIKT FSGQTHGFVH RKREDCSPAD KPYIDEARRN LIEWLNKYM
• Purity: >95% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: CMBL carboxymethylenebutenolidase homolog (Pseudomonas) [ Homo sapiens ]
• Official Symbol: CMBL
• Synonyms: CMBL; carboxymethylenebutenolidase homolog (Pseudomonas); carboxymethylenebutenolidase like (Pseudomonas); carboxymethylenebutenolidase homolog; FLJ23617; carboxymethylenebutenolidase-like (Pseudomonas); JS-1
• Gene ID: 134147
• mRNA Refseq: NM_138809
• Protein Refseq: NP_620164
• MIM: 613379
• UniProt ID: Q96DG6
• Chromosome Location: 5p15.2
• Pathway: Metabolic pathways, organism-specific biosystem
• Function: hydrolase activity