Recombinant Human CARD11 Protein, Myc/DDK-tagged

• Cat.No.: CARD11-01H
• Product Overview: Purified recombinant protein of Homo sapiens caspase recruitment domain family, member 11 (CARD11) with a C-Myc/DDK tag was expressed in HEK293T.

Specification

• Description: The protein encoded by this gene belongs to the membrane-associated guanylate kinase (MAGUK) family, a class of proteins that functions as molecular scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. This protein is also a member of the CARD protein family, which is defined by carrying a characteristic caspase-associated recruitment domain (CARD). This protein has a domain structure similar to that of CARD14 protein. The CARD domains of both proteins have been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-kappaB activation. When expressed in cells, this protein activated NF-kappaB and induced the phosphorylation of BCL10.
• Source: HEK293T
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 133.1 kDa
• AA Sequence: MPGGGPEMDDYMETLKDEEDALWEN VECNRHMLSRYINPAKLTPYLRQCK VIDEQDEDEVLNAPMLPSKINRAGR LLDILHTKGQRGYVVFLESLEFYYP ELYKLVTGKEPTRRFSTIVVEEGHE GLTHFLMNEVIKLQQQMKAKDLQRC ELLARLRQLEDEKKQMTLTRVELLT FQERYYKMKEERDSYNDELVKVKDD NYNLAMRYAQLSEEKNMAVMRSRDL QLEIDQLKHRLNKMEEECKLERNQS LKLKNDIENRPKKEQVLELERENEM LKTKNQELQSIIQAGKRSLPDSDKA ILDILEHDRKEALEDRQELVNRIYN LQEEARQAEELRDKYLEEKEDLELK CSTLGKDCEMYKHRMNTVMLQLEEV ERERDQAFHSRDEAQTQYSQCLIEK DKYRKQIRELEEKNDEMRIEMVRRE ACIVNLESKLRRLSKDSNNLDQSLP RNLPVTIISQDFGDASPRTNGQEAD DSSTSEESPEDSKYFLPYHPPQRRM NLKGIQLQRAKSPISLKRTSDFQAK GHEEEGTDASPSSCGSLPITNSFTK MQPPRSRSSIMSITAEPPGNDSIVR RYKEDAPHRSTVEEDNDSGGFDALD LDDDSHERYSFGPSSIHSSSSSHQS EGLDAYDLEQVNLMFRKFSLERPFR PSVTSVGHVRGPGPSVQHTTLNGDS LTSQLTLLGGNARGSFVHSVKPGSL AEKAGLREGHQLLLLEGCIRGERQS VPLDTCTKEEAHWTIQRCSGPVTLH YKVNHEGYRKLVKDMEDGLITSGDS FYIRLNLNISSQLDACTMSLKCDDV VHVRDTMYQDRHEWLCARVDPFTDH DLDMGTIPSYSRAQQLLLVKLQRLM HRGSREEVDGTHHTLRALRNTLQPE EALSTSDPRVSPRLSRASFLFGQLL QFVSRSENKYKRMNSNERVRIISGS PLGSLARSSLDATKLLTEKQEELDP ESELGKNLSLIPYSLVRAFYCERRR PVLFTPTVLAKTLVQRLLNSGGAME FTICKSDIVTRDEFLRRQKTETIIY SREKNPNAFECIAPANIEAVAAKNK HCLLEAGIGCTRDLIKSNIYPIVLF IRVCEKNIKRFRKLLPRPETEEEFL RVCRLKEKELEALPCLYATVEPDMW GSVEELLRVVKDKIGEEQRKTIWVD EDQLSGPTRTRPLEQKLISEEDLAA NDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

Gene Information

• Gene Name: CARD11 caspase recruitment domain family member 11 [ Homo sapiens (human) ]
• Official Symbol: CARD11 caspase recruitment domain family member 11 [ Homo sapiens (human) ]
• Synonyms: CARD11; caspase recruitment domain family member 11; PPBL; BENTA; BIMP3; IMD11; CARMA1; IMD11A; caspase recruitment domain-containing protein 11; CARD-containing MAGUK protein 1; bcl10-interacting maguk protein 3; carma 1
• Gene ID: 84433
• mRNA Refseq: NM_032415
• Protein Refseq: NP_115791
• MIM: 607210
• UniProt ID: Q9BXL7