Recombinant Human CARS Protein, GST-Tagged

• Cat.No.: CARS-0403H
• Product Overview: Human CARS full-length ORF (AAH02880, 1 a.a. - 748 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: This gene encodes a class 1 aminoacyl-tRNA synthetase, cysteinyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. This gene is one of several located near the imprinted gene domain on chromosome 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian and breast cancers. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Aug 2010]
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Molecular Mass: 107.91 kDa
• AA Sequence: MADSSGQQGKGRRVQPQWSPPAGTQ PCRLHLYNSLTRNKEVFIPQDGKKV TWYCCGPTVYDASHMGHARSYISFD ILRRVLKDYFKFDVFYCMNITDIDD KIIKRARQNHLFEQYREKRPEAAQL LEDVQAALKPFSVKLNETTDPDKKQ MLERIQHAVQLATEPLEKAVQSRLT GEEVNSCVEVLLEEAKDLLSDWLDS TLGCDVTDNSIFSKLPKFWEGDFHR DMEALNVLPPDVLTRVSEYVPEIVN FVQKIVDNGYGYVSNGSVYFDTAKF ASSEKHSYGKLVPEAVGDQKALQEG EGDLSISADRLSEKRSPNDFALWKA SKPGEPSWPCPWGKGRPGWHIECSA MAGTLLGASMDIHGGGFDLRFPHHD NELAQSEAYFENDCWVRYFLHTGHL TIAGCKMSKSLKNFITIKDALKKHS ARQLRLAFLMHSWKDTLDYSSNTME SALQYEKFLNEFFLNVKDILRAPVD ITGQFEKWGEEEAELNKNFYDKKTA IHKALCDNVDTRTVMEEMRALVSQC NLYMAARKAVRKRPNQALLENIALY LTHMLKIFGAVEEDSSLGFPVGGPG TSLSLEATVMPYLQVLSEFREGVRK IAREQKVPEILQLSDALRDNILPEL GVRFEDHEGLPTVVKLVDRNTLLKE REEKRRVEEEKRKKKEEAARRKQEQ EAAKLAKMKIPPSEMFLSETDKYSK FDENGLPTHDMEGKELSKGQAKKLK KLFEAQEKLYKEYLQMAQNGSFQ
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: CARS cysteinyl-tRNA synthetase [ Homo sapiens ]
• Official Symbol: CARS
• Synonyms: CARS; cysteinyl-tRNA synthetase; cysteine--tRNA ligase, cytoplasmic; CARS1; cysteine tRNA ligase 1; cytoplasmic; cysteine translase; cysteine tRNA ligase 1, cytoplasmic; CYSRS; MGC:11246
• Gene ID: 833
• mRNA Refseq: NM_001014437
• Protein Refseq: NP_001014437
• MIM: 123859
• UniProt ID: P49589