Recombinant Human casein kinase 2, alpha 1 polypeptide

• Cat.No.: CSNK2A1-21H
• Product Overview: Recombinant Human Casein Kinase 2 alpha is a non-glycosylated polypeptide having a molecular mass of 45.1 kDa. Casein Kinase 2 is purified by proprietary chromatographic techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: The Casein kinase 2 (EC 2.7.11.1) is a serine/threonine-selective protein kinase that is a tetramer of two alpha subunits and two beta subunits. The alpha subunits have the catalytic kinase domain. Casein kinase 2 has been implicated in cell cycle control, DNA repair, regulation of the circadian rhythm and other cellular processes. Casein kinase 2 activity has been reported to be activated following Wnt signaling pathway activation. A Pertussis toxin-sensitive G protein and Disheveled appear to be an intermediary between Wnt-mediated activation of the Frizzled receptor and activation of casein kinase 2. Mice that lack casein kinase 2 alpha prime have a defect in the morphology of developing sperm.
• Unit Definition: No protease activity detectable, specific activity > 1.320 U/mg (1U = 1µmol/min at 37 degree C) using the synthetic peptide RRRDDDSDDD.
• Physical Appearance: Sterile Filtered clear solution.
• Purity: Greater than 99% as determined by SDS-PAGE.
• Formulation: CK2 (0.4 mg/ml) is supplied in 25mM Tris-HCl, 500mM NaCl, 1mM DTT, 500μM PMSF, 50% glycerol, pH 8.5.
• Storage: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.

Gene Information

• Gene Name: CSNK2A1 casein kinase 2, alpha 1 polypeptide [ Homo sapiens ]
• Synonyms: CSNK2A1; casein kinase 2, alpha 1 polypeptide; CKII; CK2A1; casein kinase II alpha 1 subunit; protein kinase CK2; CK2 catalytic subunit alpha; EC 2.7.11.1; OTTHUMP00000029925; OTTHUMP00000029926
• Gene ID: 1457
• mRNA Refseq: NM_001895
• Protein Refseq: NP_001886
• MIM: 115440
• UniProt ID: P68400
• Chromosome Location: 20p13
• Pathway: Adherens junction; Tight junction; Wnt signaling pathway
• Function: ATP binding; nucleotide binding; protein N-terminus binding; protein serine/threonine kinase activity; transferase activity