Recombinant Human Caspase 10, Apoptosis-related Cysteine Peptidase

• Cat.No.: CASP10-598H

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: Caspase-10 (also known as Mch4 and Flice2) is a member of the interleukin-1b converting enzyme (ICE) family of cysteine proteases. Similar as other caspases, caspase-10 exists in cells as an inactive proenzyme. During apoptosis, procaspase-10 is processed at aspartate residues by self-proteolysis and/or cleavage by upstream caspases. The processed form of caspase-10 consists of large (18kDa) and small (11kDa) subunits which associate to form the active enzyme.
• Sourse/Host: E. coli.
• Formulation: Lyophilized.
• Reconstitution: Reconstitute with PBS to 1U/µl.
• Specific Activity: ~15"000U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate IETD-pNA per hour at 37°C in a reaction solution containing 50mM HEPES, pH 7.2, 50mM NaCl, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT.
• Purity: ≥90% (SDS-PAGE).
• Storage: For long term storage, -80°C.
• Pathways: Apoptosis; RIG-I-like receptor signaling pathway

Gene Information

• Gene Name: CASP10 caspase 10, apoptosis-related cysteine peptidase [ Homo sapiens ]
• Synonyms: caspase 10, apoptosis-related cysteine peptidase; MCH4; ALPS2; FLICE2; CASP10; caspase-10; CASP-10; FADD-like ICE2; apoptotic protease MCH-4; ICE-like apoptotic protease 4; interleukin-1B-converting enzyme 2; caspase 10, apoptosis-related cysteine protease; FAS-associated death domain protein interleukin-1B-converting enzyme 2;Apoptotic protease Mch-4; Fas-associated death domain protein; EC 3.4.22.63
• Gene ID: 843
• mRNA Refseq: NM_001230
• Protein Refseq: NP_001221
• MIM: 601762
• UniProt ID: Q92851
• Chromosome Location: 2q33-q34
• Function: cysteine-type endopeptidase activity; identical protein binding; peptidase activity; protein binding