Recombinant Human CBFB Protein, GST-Tagged

• Cat.No.: CBFB-0455H
• Product Overview: Human CBFB full-length ORF (AAH18509, 1 a.a. - 182 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: The protein encoded by this gene is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2). The beta subunit is a non-DNA binding regulatory subunit; it allosterically enhances DNA binding by alpha subunit as the complex binds to the core site of various enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers and GM-CSF promoters. Alternative splicing generates two mRNA variants, each encoding a distinct carboxyl terminus. In some cases, a pericentric inversion of chromosome 16 [inv(16)(p13q22)] produces a chimeric transcript consisting of the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain 11. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Molecular Mass: 45.76 kDa
• AA Sequence: MPRVVPDQRSKFENEEFFRKLSREC EIKYTGFGDRPHEERQARLQNACRD GRSEIAFVATGTNLSLQFFPASWQG EQRQTPSREYVDLEREAGKVYLKAP MILNGVCVIWKGWIDLQRLDGMGCL EFDEERAQQEDALAQQAFEEARRRT REFEDRDRSHREEMEVRVSQLLAVT GKKTTRP
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: CBFB core-binding factor, beta subunit [ Homo sapiens ]
• Official Symbol: CBFB
• Synonyms: CBFB; core-binding factor, beta subunit; core-binding factor subunit beta; PEBP2B; CBF-beta; PEA2-beta; PEBP2-beta; SL3-3 enhancer factor 1 beta subunit; SL3-3 enhancer factor 1 subunit beta; SL3/AKV core-binding factor beta subunit; polyomavirus enhancer-binding protein 2 beta subunit; polyomavirus enhancer binding protein 2, beta subunit
• Gene ID: 865
• mRNA Refseq: NM_001755
• Protein Refseq: NP_001746
• MIM: 121360
• UniProt ID: Q13951