Recombinant Human CCAAT/enhancer binding protein Alpha, His-tagged

• Cat.No.: CEBPA-73H
• Product Overview: Recombinant Human CCAAT/enhancer binding protein(C/EBP) Alpha, was expressed in Escherichia Coli, amino acids 126 (aa 270-358), His-Tag fusion protein

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: amino acids 126 (aa 270-358)
• Description: CCAAT/enhancer binding protein(C/EBP) a is a family of transcription factors that all contain a highly conserved, C/EBP family consist of several related proteins, C/EBP a,b,g,d, that form homodimers and that form heterodimers with each other. C/EBP proteins contain the bZIP region, which is characterized by two motifs in the C-terminal half of the protein; a basic region involved in DNA binding and a leucine zipper motif involved in dimerization.
• Molecular Mass: 14.5 kDa
• Purity: Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Applications: ELISA, Western, antibody blocking
• Usage: This item is for LABORATORY RESEARCH USE ONLY.
• Storage: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). If supplied in powder then reconstitute it in 100ul water for 1mg/mL stock and store in liquid at 4°C for ~ 1 week or aliquots in suitable size and store at -20°C for long term storage.
• Storage Buffer: The protein contains 20mM Tris-HCl pH7.5, 0.1M NaCl and 5mM b-Mercaptoethanol.
• Dilutions: Users must optimize the appropriate concentration and conditions for each assay.

Gene Information

• Gene Name: CEBPA CCAAT/enhancer binding protein (C/EBP), alpha [ Homo sapiens ]
• Official Symbol: CEBPA
• Synonyms: CEBP; C/EBP-alpha; CCAAT/enhancer-binding protein alpha
• Gene ID: 1050
• mRNA Refseq: NM_001285829
• Protein Refseq: NP_001272758
• MIM: 116897
• Chromosome Location: 19q13.1
• Pathway: Acute myeloid leukemia, organism-specific biosystem; Adipogenesis, organism-specific biosystem; E2F transcription factor network, organism-specific biosystem
• Function: DNA binding; HMG box domain binding; protein binding