Recombinant Human CD147, GST-tagged

• Cat.No.: CD147-10906H
• Product Overview: Recombinant Human CD147 protein, fused to GST-tag, was expressed in E.coli and purified by GSH-sepharose.

Specification

• Species: Human
• Source: E.coli
• Tag: GST
• Protein Length: N-term-269a.a.
• Description: This gene encodes a G-protein coupled receptor that binds non-sulfated members of the cholecystokinin (CCK) family of peptide hormones. This receptor is a major physiologic mediator of pancreatic enzyme secretion and smooth muscle contraction of the gallbladder and stomach. In the central and peripheral nervous system this receptor regulates satiety and the release of beta-endorphin and dopamine.
• Storage: The protein is stored in PBS buffer at -20℃. Avoid repeated freezing and thawing cycles.
• Storage Buffer: 1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 100mM GSH and 1% Triton X-100,15%glycerol.

Gene Information

• Gene Name: BSG basigin (Ok blood group) [ Homo sapiens ]
• Official Symbol: BSG
• Synonyms: BSG; basigin (Ok blood group); basigin (OK blood group) , OK; basigin; CD147; EMMPRIN; CD147 antigen; OK blood group antigen; collagenase stimulatory factor; leukocyte activation antigen M6; extracellular matrix metalloproteinase inducer; tumor cell-derived collagenase stimulatory factor; M6; OK; 5F7; TCSF
• Gene ID: 682
• mRNA Refseq: NM_001728
• Protein Refseq: NP_001719
• MIM: 109480
• UniProt ID: P35613
• Chromosome Location: 19p13.3
• Pathway: Basigin interactions, organism-specific biosystem; Cell surface interactions at the vascular wall, organism-specific biosystem; Hemostasis, organism-specific biosystem; Integrin cell surface interactions, organism-specific biosystem; Matrix Metalloproteinases, organism-specific biosystem; Metabolism, organism-specific biosystem; Pyruvate metabolism, organism-specific biosystem
• Function: lactate transmembrane transporter activity; mannose binding; sugar binding

Citation

Novel Antibody Exerts Antitumor Effect through Downregulation of CD147 and Activation of Multiple Stress Signals

Journal: Journal of Oncology    PubMed ID: 36385956    Data: 2022/12/14

Authors: Keisuke Fukuchi, Kayoko Nanai, Toshinori Agatsuma

Article Snippet:Hybridomas producing the monoclonal antibody were generated from mice or rats after immunization with recombinant human CD147 protein (Creative BioMart, Shirley, NY, US) or CD147-expressing cells by the myeloma fusion method.. Antibodies showing binding activity to recombinant CD147 protein were screened by the ELISA.Antibodies showing binding activity to recombinant CD147 protein were screened by the ELISA.

Antibodies used for flow cytometric analysis.

Antibodies used for capillary electrophoresis-based western blot analysis.

Antibody profile of the anti-CD147 antibody, h4 # 147D.