Recombinant Human CD3E & CD3D Protein, Fc-His-Avi &Fc-Flag-Avi-tagged, Biotinylated
Cat.No. : | CD3E & CD3D-567H |
Product Overview : | Biotinylated Human CD3E&CD3D Heterodimer Protein, Fc,His,Avitag&Fc,Flag,Avitag is expressed from human HEK293 cells. It contains AA Asp 23 - Asp 126 (CD3E) & Phe 22 - Ala 105 (CD3D) (Accession # P07766-1(CD3E) & P04234-1(CD3D)). It is the biotinylated form of Human CD3 epsilon & CD3 delta Protein. |
- Specification
- Gene Information
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Description : | T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. |
Source : | HEK293 |
Species : | Human |
Tag : | Fc-His-Avi &Fc-Flag-Avi |
Predicted N Terminal : | Asp 23 (CD3E) & Phe 22 (CD3D) |
Form : | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. |
Molecular Mass : | Biotinylated Human CD3E&CD3D Heterodimer Protein, Fc,His,Avitag&Fc,Flag,Avitag is produced by co-expression of CD3E and CD3D, has a calculated MW of 45.1kDa (CD3E) & 42.6 kDa (CD3D). Subunit CD3E is fused with a human IgG1 Fc fragment at the C-terminus, followed by an Avi tag, the polyhistidine tag is inserted in-between the Fc and Avi tags at the C-terminus and subunit CD3D contains a human IgG1 Fc fragment at the C-terminus, followed by an Avi tag, the flag tag is inserted in-between the Fc and Avi tags at the C-terminus. As a result of glycosylation, the heterodimer protein migrates as 50-60 kDa under reducing (R) condition. |
Protein length : | Asp 23 - Asp 126 (CD3E) & Phe 22 - Ala 105 (CD3D) |
Endotoxin : | Less than 1.0 EU per μg by the LAL method. |
Purity : | >90% as determined by SDS-PAGE. >90% as determined by SEC-MALS. |
Storage : | For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents. |
Gene Name : | CD3E CD3e molecule, epsilon (CD3-TCR complex) [ Homo sapiens ] |
Official Symbol : | CD3E & CD3D |
Synonyms : | CD3E; CD3D |
Gene ID : | 916 |
mRNA Refseq : | NM_000733 |
Protein Refseq : | NP_000724 |
UniProt ID : | P07766 |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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