Recombinant Human CD3E & CD3D protein, hFc-tagged

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Recombinant Human CD3E & CD3D protein, hFc-tagged

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Cat.No. : CD3E & CD3D-109H
Product Overview : Human CD3 epsilon & CD3 delta Heterodimer Protein is expressed from mammalian with a Fc at the Cterminal. It contains Asp 23 - Asp 126 (CD3E) & Phe22-Ala105 (CD3D).
Description : T-cell surface glycoprotein CD3 epsilon & CD3 delta chain, also known as CD3E & CD3D , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
Source : HEK293
Species : Human
Tag : hFc
Form : Supplied as 0.22um filtered solution in PBS (pH 7.4).
Molecular Mass : The protein has a calculated MW of 37.8 kDa (CD3E) and 35.4 kDa (CD3D).As a result of glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
Protein length : It contains Asp 23 - Asp 126 (CD3E) & Phe22-Ala105 (CD3D).
Endotoxin : Less than 0.01EU per ug by the LAL method.
Purity : > 95% as determined by Bis-Tris PAGE
Storage : The product should be stored at -70°C. Please do not repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Tris-Bis PAGE :

HPLC data :

ELISA data :

Gene Name : CD3E CD3e molecule, epsilon (CD3-TCR complex) [ Homo sapiens ]
Official Symbol : CD3E ; CD3D
Synonyms : CD3;CD3D;CD3-DELTA;T3D;CD3E
Gene ID : 916
mRNA Refseq : NM_000733
Protein Refseq : NP_000724
UniProt ID : P07766

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