Recombinant Human CD3E & CD3D protein, hFc-tagged

• Cat.No.: CD3E & CD3D-109H
• Product Overview: Human CD3 epsilon & CD3 delta Heterodimer Protein is expressed from mammalian with a Fc at the Cterminal. It contains Asp 23 - Asp 126 (CD3E) & Phe22-Ala105 (CD3D).

Specification

• Description: T-cell surface glycoprotein CD3 epsilon & CD3 delta chain, also known as CD3E & CD3D , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
• Source: HEK293
• Species: Human
• Tag: hFc
• Form: Supplied as 0.22um filtered solution in PBS (pH 7.4).
• Molecular Mass: The protein has a calculated MW of 37.8 kDa (CD3E) and 35.4 kDa (CD3D).As a result of glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
• Protein length: It contains Asp 23 - Asp 126 (CD3E) & Phe22-Ala105 (CD3D).
• Endotoxin: Less than 0.01EU per ug by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE
• Storage: The product should be stored at -70°C. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: CD3E CD3e molecule, epsilon (CD3-TCR complex) [ Homo sapiens ]
• Official Symbol: CD3E ; CD3D
• Synonyms: CD3;CD3D;CD3-DELTA;T3D;CD3E
• Gene ID: 916
• mRNA Refseq: NM_000733
• Protein Refseq: NP_000724
• UniProt ID: P07766