Active Recombinant Human CD3E & CD3D protein, His & Flag-tagged, Biotinylated
Cat.No. : | CD3E & CD3D-375H |
Product Overview : | Biotinylated Human CD3 epsilon & CD3 delta is expressed from human HEK293 cells. It contains AA Asp 23 - Asp 126 (CD3E) & Phe 22 - Ala 105 (CD3D) (Accession # NP_000724.1 (CD3E) & AAH39035 (CD3D)). It is the biotinylated form of Human CD3 epsilon & CD3 delta Protein. The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product. The biotin to protein ratio is 2.5-4.5 as determined by the HABA assay. |
- Specification
- Gene Information
- Related Products
Source : | HEK293 |
Species : | Human |
Tag : | His & Flag |
Form : | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. |
Bio-activity : | Immobilized Biotinylated Human CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag, primary amine labeling at 1 μg/mL (100 μL/well)on streptavidin precoated (0.2 μg/well) plate, can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3), premium grade with a linear range of 2-20 ng/mL. |
Molecular Mass : | The protein has a calculated MW of 39.2 kDa (CD3E) and 37.5 kDa (CD3D) . The protein migrates as 45-55 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation. |
Protein Length : | Asp 23 - Asp 126 (CD3E) & Phe 22 - Ala 105 (CD3D) |
Endotoxin : | Less than 1.0 EU per μg by the LAL method. |
Purity : | >95% as determined by SDS-PAGE. >90% as determined by SEC-MALS. |
Storage : | For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20 centigrade to -70 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents. |
Gene Name : | CD3E CD3e molecule, epsilon (CD3-TCR complex) [ Homo sapiens ] |
Official Symbol : | CD3E |
Synonyms : | CD3E; CD3e molecule, epsilon (CD3-TCR complex); CD3e antigen, epsilon polypeptide (TiT3 complex); T-cell surface glycoprotein CD3 epsilon chain; CD3-epsilon; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell antigen receptor complex, epsilon subunit of T3; T3E; TCRE; FLJ18683; |
Gene ID : | 916 |
mRNA Refseq : | NM_000733 |
Protein Refseq : | NP_000724 |
UniProt ID : | P07766 |
Products Types
◆ Recombinant Protein | ||
CD3E-32H | Recombinant Human CD3E Protein, C-Fc-Avi tagged, Biotinylated | +Inquiry |
CD3E-143H | Recombinant Human CD3E Protein, Strep-tagged | +Inquiry |
CD3E-0803H | Recombinant Human CD3E Protein, GST-Tagged | +Inquiry |
CD3E-321H | Recombinant Human CD3E Protein, His-tagged | +Inquiry |
CD3E-1085M | Recombinant Mouse CD3E Protein (Met1-Asp108), His-Fc-tagged, Biotinylated | +Inquiry |
◆ Lysates | ||
CD3E-1602CCL | Recombinant Cynomolgus CD3E cell lysate | +Inquiry |
CD3E-1275HCL | Recombinant Human CD3E cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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