Recombinant Human CD3E & CD3G protein(Met1-Asp126 & Met1-Ser113), LFc-tagged

• Cat.No.: CD3E & CD3G-3256H
• Product Overview: Recombinant Human CD3E (NP_000724.1) (Met1-Asp126) was expressed in HEK293, fused with the Fc region of Llama IgG2b at the C-terminus, constructed the plasmid 1; Recombinant Human CD3G (NP_000064.1) (Met1-Ser113) was fused with the Fc region of Llama IgG2b at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the Human CD3E/CD3G heterodimer was purified.

Specification

• Source: HEK293
• Species: Human
• Tag: C-LFc
• Protein length: Met1-Asp126 & Met1-Ser113
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human CD3E/CD3G consists of 696 (353+343) amino acids and has a calculated molecular mass of 78.4 (39.7+38.7) KDa.
• Endotoxin: < 0.01 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Reviews

03/22/2023

Manufacturers with the capacity for bulk production can offer CD3E protein in larger quantities, which is advantageous for researchers working on large-scale experiments or collaborations.

09/12/2021

bulk production often leads to economies of scale, making the CD3E protein more cost-effective for research budgets.

06/17/2018

Manufacturers can actively seek collaborations and partnerships with researchers to develop new applications or optimize methodologies using CD3E protein.

Q&As

Can CD3E be used to assess the effectiveness of cancer immunotherapies?

Yes, CD3E levels and T-cell function can be monitored to evaluate the response to cancer immunotherapies like checkpoint inhibitors.

Are there any CD3E-targeted therapies?

There are currently no approved therapies that directly target CD3E, but it is a target for research in developing immunotherapies and cancer treatments.

Are there any ongoing clinical trials involving CD3E?

There may be ongoing clinical trials using CD3E-targeted therapies in the context of cancer and autoimmune diseases. It's important to check clinical trial databases for specific studies.

Can CD3E levels be used to monitor the progression of HIV?

Yes, monitoring CD3E levels in HIV-infected individuals can provide valuable information about the progression of the disease and the effectiveness of antiretroviral therapy.

How is CD3E used in allergy diagnostics?

CD3E can be used in research to study T-cell responses in allergic conditions, helping to better understand and diagnose allergies.