Recombinant Human CD3E protein(C119S, C122S), hFc-tagged

• Cat.No.: CD3E-674H
• Product Overview: Recombinant Human CD3E protein(C119S, C122S)(NP_000724.1)(Asp23-Asp126), fused with C-terminal hFc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: Asp23-Asp126 (C119S, C122S)
• Form: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4).
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Bio-activity: Immobilized Human CD3E, hFc Tag at 2 μg/ml (100 μl/Well) on the plate. Dose response curve for Biotinylated Anti-CD3 Antibody, hFc Tag with the EC50 of 58.3ng/ml determined by ELISA.
• Molecular Mass: It has a predicted MW of 36.1 kDa. Due to glycosylation, the protein migrates to 35-55 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 1 EU per μg by the LAL method.
• Purity: >95% as determined by Bis-Tris PAGE

Gene Information

• Official Symbol: CD3E
• Synonyms: CD3E; CD3e molecule, epsilon (CD3-TCR complex); CD3e antigen, epsilon polypeptide (TiT3 complex); T-cell surface glycoprotein CD3 epsilon chain; CD3-epsilon; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell antigen receptor complex, epsilon subunit of T3; T3E; TCRE; FLJ18683
• Gene ID: 916
• mRNA Refseq: NM_000733
• Protein Refseq: NP_000724
• UniProt ID: P07766