Recombinant Human CD52 protein, Fc-tagged

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Recombinant Human CD52 protein, Fc-tagged

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Cat.No. : CD52-393H
Product Overview : Recombinant Human CD52 (Gly25-Ser36) protein was fused to human IgG1 Fc tag at C-terminus and expressed in human 293 cells (HEK293).
Description : CAMPATH-1 antigen, also known as cluster of differentiation 52 (CD52), is a glycoprotein that in humans is encoded by the CD52 gene.It is widely expressed on the cell surface of immune cells, such as mature lymphocytes, natural killer cells (NK), eosinophils, neutrophils, monocytes/macrophages, and dendritic cells (DCs).ligation of cell surface CD52 molecules may offer costimulatory signals for T-cell activation and proliferation.
Source : HEK293
Species : Human
Tag : human IgG1 Fc
Predicted N Terminal : Gly25
Form : Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 27.9 kDa. The protein migrates as 38-43 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length : Gly25-Ser36
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of CD52-393H :

Human CD52, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA of CD52-393H :

Immobilized Human CD52, Fc Tag at 10 μg/mL (100 μL/well) can bind Alemtuzumab with a linear range of 0.313-10 μg/mL (QC tested).

Gene Name : CD52
Official Symbol : CD52
Synonyms : CD52; CD52 molecule; CD52 antigen (CAMPATH 1 antigen) , CDW52; CAMPATH-1 antigen; he5; cambridge pathology 1 antigen; epididymal secretory protein E5; CD52 antigen (CAMPATH-1 antigen); CDW52 antigen (CAMPATH-1 antigen); human epididymis-specific protein 5; CDW52
Gene ID : 1043
mRNA Refseq : NM_001803
Protein Refseq : NP_001794
MIM : 114280
UniProt ID : P31358
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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