Recombinant Human CDC2 Protein, GST-Tagged

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Recombinant Human CDC2 Protein, GST-Tagged

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Cat.No. : CDC2-0906H
Product Overview : Human CDC2 (NM_001786, 1 a.a. - 297 a.a.) and CCNA2 (NM_001237, 1 a.a. - 432 a.a.) recombinanat protein with GST GST tag expressed in Sf9 cells.
Description : The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
Source : Insect Cell
Species : Human
Tag : GST
Form : Liquid
Bio-activity : 8 pmol/μg x min
Molecular Mass : CDC2: 63.882, CCNA2 kDa
Applications : Functional Study
SDS-PAGE
Storage : Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing
Concentration : 0.126 μg/μL
Storage Buffer : In 50 mM Hepes, 100 mM NaCl, pH 7.5. (5 mM DTT, 15 mM reduced glutathione, 20% glycerol)
Quality Control Testing :

2 μg/lane SDS-PAGE Stained with Coomassie Blue

Result of activity analysis :

Gene Name : CDK1 cyclin dependent kinase 1 [ Homo sapiens (human) ]
Official Symbol : CDC2
Synonyms : CDK1; cyclin dependent kinase 1; CDC2; CDC28A; P34CDC2; cyclin-dependent kinase 1; cell cycle controller CDC2; cell division control protein 2 homolog; cell division cycle 2, G1 to S and G2 to M; cell division protein kinase 1; p34 protein kinase; EC 2.7.11.22; EC 2.7.11.23; Cyclin Dependent Kinase 1; Cell Division Cycle 2, G1 To S And G2 To M; Cell Division Control Protein 2 Homolog; Cell Division Protein Kinase 1; P34 Protein Kinase; Cell Cycle Controller CDC2; Cyclin-Dependent Kinase 1
Gene ID : 983
mRNA Refseq : NM_001170406
Protein Refseq : NP_001163877
MIM : 116940
UniProt ID : P06493

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