Recombinant Human CDC26, His-tagged

• Cat.No.: CDC26-3554H
• Product Overview: Recombinant humanCDC26 protein, fused to His-tag at N-terminus, was expressed in E. coli andpurified by using conventional chromatography.

Specification

• Species: Human
• Source: Human
• Tag: His
• Description: CDC26,also known as ANAPC12, APC12, belongs to the CDC26 family which orchestrates progressionthrough mitosis by decorating cell-cycle regulators with ubiquitin chains.This protein is component of the anaphase promoting complex/cyclosome (APC/C),a cell cycle-regulated E3 ubiquitin ligase that controls progression throughmitosis and the G1 phase of the cell cycle. The APC/C complex acts bymediating ubiquitination and subsequent degradation of target proteins.
• Form: Liquid. 20mM Tris-HClbuffer (pH8.0) containing 40% glycerol, 0.1M NaCl.
• Molecular Weight: 11.9 kDa (105 aa), confirmed by MALDI-TOF.
• Purity: > 90%by SDS - PAGE
• Concentration: 1 mg/ml(determined by Bradford assay)
• Sequences of aminoacids: MGSSHHHHHH SSGLVPRGSH MLRRKPTRLELK LDDIEEFE NIRKDLETRK KQKEDVEVVG GSDGEGAIGL SSDPKSREQM INDRIGYKPQ PKPNNRSSQFGSLEF
• Storage: Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: CDC26 cell division cycle 26homolog (S. cerevisiae) [ Homo sapiens ]
• Official Symbol: CDC26
• Synonyms: CDC26; cell division cycle 26 homolog (S.cerevisiae); APC12; ANAPC12; C9orf17; anaphase-promoting complex subunitCDC26; anaphase promoting complex subunit 12; anaphase-promoting complexsubunit 12; cell division cycle protein 26 homolog; CDC26 subunit of anaphasepromoting complex; OTTHUMP00000021952
• Gene ID: 246184
• mRNA Refseq: NM_139286
• Protein Refseq: NP_644815
• UniProt ID: Q8NHZ8
• Chromosome Location: 9q32
• Pathway: APC/C complex; APC/C-mediated degradationof cell cycle proteins; Adaptive Immune System; Antigen processing:Ubiquitination & Proteasome degradation; Cell Cycle; Class I MHC mediatedantigen processing & presentation; HTLV-I infection; Immune System;Mitotic Spindle Checkpoint; Oocyte meiosis; Phosphorylation of the APC/C;Progesterone-mediated oocyte maturation; Regulation of mitotic cell cycle;Ubiquitin mediated proteolysis
• Function: protein binding