Recombinant Human CDC34 protein

• Cat.No.: CDC34-220H
• Product Overview: Recombinant Human CDC34 protein was expressed in E.coli.

Specification

• Description: The protein encoded by this gene is a member of the ubiquitin-conjugating enzyme family. Ubiquitin-conjugating enzyme catalyzes the covalent attachment of ubiquitin to other proteins. This protein is a part of the large multiprotein complex, which is required for ubiquitin-mediated degradation of cell cycle G1 regulators, and for the initiation of DNA replication.
• Source: E.coli
• Species: Human
• Form: A 0.2 µm filtered concentrated solution in 50 mM HEPES, pH 7.0, with 125 mM NaCl, 10 % Glycerol, 5 % Trehalose, 1 mM DTT.
• Molecular Mass: Approximately 26.7 kDa, a single non-glycosylated polypeptide chain containing 236 amino acids.
• Protein length: 236
• AA Sequence: MARPLVPSSQKALLLELKGLQEEPV EGFRVTLVDEGDLYNWEVAIFGPPN TYYEGGYFKARLKFPIDYPYSPPAF RFLTKMWHPNIYETGDVCISILHPP VDDPQSGELPSERWNPTQNVRTILL SVISLLNEPNTFSPANVDASVMYRK WKESKGKDREYTDIIRKQVLGTKVD AERDGVKVPTTLAEYCVKTKAPAPD EGSDLFYDDYYEDGEVEEEADSCFG DDEDDSGTEES
• Endotoxin: Less than 0.1 EU/µg of rHuUBE2R1 as determined by LAL method.
• Purity: >95% by SDS-PAGE and HPLC analyses.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 centigrade as supplied. 3 months, -20 to -70 centigrade under sterile conditions after opening.

Gene Information

• Gene Name: CDC34
• Official Symbol: CDC34
• Synonyms: CDC34; cell division cycle 34 homolog (S. cerevisiae); cell division cycle 34; ubiquitin-conjugating enzyme E2 R1; E2 CDC34; UBC3; UBE2R1; ubiquitin carrier protein; ubiquitin-protein ligase R1; ubiquitin-conjugating enzyme E2-CDC34; ubiquitin-conjugating enzyme E2-32 KDA complementing; UBCH3; E2-CDC34
• Gene ID: 997
• mRNA Refseq: NM_004359
• Protein Refseq: NP_004350
• MIM: 116948
• UniProt ID: P49427