Recombinant Human CDC34 protein

• Cat.No.: CDC34-220H
• Product Overview: Recombinant Human CDC34 protein(P49427)(1-236 aa) was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Protein Length: 1-236 aa
• Form: A 0.2 μm filtered concentrated solution in 50 mM HEPES, pH 7.0, with 125 mM NaCl, 10 % Glycerol, 5 % Trehalose, 1 mM DTT.
• Molecular Mass: Approximately 26.7 kDa, a single non-glycosylated polypeptide chain containing 236 amino acids.
• AASequence: MARPLVPSSQ KALLLELKGL QEEPVEGFRV TLVDEGDLYN WEVAIFGPPN TYYEGGYFKA RLKFPIDYPY SPPAFRFLTK MWHPNIYETG DVCISILHPP VDDPQSGELP SERWNPTQNV RTILLSVISL LNEPNTFSPA NVDASVMYRK WKESKGKDRE YTDIIRKQVL GTKVDAERDG VKVPTTLAEY CVKTKAPAPD EGSDLFYDDY YEDGEVEEEA DSCFGDDEDD SGTEES
• Endotoxin: Less than 0.1 EU/µg of rHuUBE2R1 as determined by LAL method.
• Purity: >95% by SDS-PAGE and HPLC analyses.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Gene Information

• Gene Name: CDC34 cell division cycle 34 homolog (S. cerevisiae) [ Homo sapiens ]
• Official Symbol: CDC34
• Synonyms: CDC34; cell division cycle 34 homolog (S. cerevisiae); cell division cycle 34; ubiquitin-conjugating enzyme E2 R1; E2 CDC34; UBC3; UBE2R1; ubiquitin carrier protein; ubiquitin-protein ligase R1; ubiquitin-conjugating enzyme E2-CDC34; ubiquitin-conjugating enzyme E2-32 KDA complementing; UBCH3; E2-CDC34
• Gene ID: 997
• mRNA Refseq: NM_004359
• Protein Refseq: NP_004350
• MIM: 116948
• UniProt ID: P49427