Active Recombinant Human CDCP1 protein, His-tagged, Low Endotoxin

• Cat.No.: CDCP1-557H
• Product Overview: Recombinant Human CDCP1 protein(Q9H5V8-1)(Phe30-Arg368 & Lys369-Thr667), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 30-368 aa& 369-667 aa
• Form: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human CDCP1 ATF&CTF, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-CDCP1 Antibody, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 74.70 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: Store at -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: CDCP1 CUB domain containing protein 1 [ Homo sapiens ]
• Official Symbol: CDCP1
• Synonyms: CDCP1; CUB domain containing protein 1; CUB domain-containing protein 1; CD318; SIMA135; membrane glycoprotein gp140; transmembrane and associated with src kinases; subtractive immunization M plus HEp3-associated 135 kDa protein; TRASK
• Gene ID: 64866
• mRNA Refseq: NM_022842
• Protein Refseq: NP_073753
• MIM: 611735
• UniProt ID: Q9H5V8