Recombinant Human CDK12 protein, GST-tagged

• Cat.No.: CDK12-16H
• Product Overview: Recombinant Human CDK12(658-end) fused with GST tag at N-terminal was expressed in Insect cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST
• Protein Length: 658-end a.a.
• Form: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Molecular Mass: ~135kDa
• Purity: > 70%
• Applications: Western Blot
• Stability: 1 year at -70 centigrade from the date of shipment
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.5μg/μl

Gene Information

• Gene Name: CDK12 cyclin-dependent kinase 12 [ Homo sapiens ]
• Official Symbol: CDK12
• Synonyms: CDK12; cyclin-dependent kinase 12; Cdc2 related kinase, arginine/serine rich , CRKRS; CDC2 related protein kinase 7; CRK7; CRKR; KIAA0904; CDC2-related protein kinase 7; cell division protein kinase 12; Cdc2-related kinase, arginine/serine-rich; cell division cycle 2-related protein kinase 7; CRKRS; hCDK12
• Gene ID: 51755
• mRNA Refseq: NM_016507
• Protein Refseq: NP_057591
• UniProt ID: Q9NYV4
• Chromosome Location: 17q12
• Function: ATP binding; RNA polymerase II carboxy-terminal domain kinase activity; cyclin binding; cyclin-dependent protein kinase activity; nucleotide binding; protein binding; protein kinase activity

Q&As

I would like to know CDK12-16H. Could you tell me the protein production/purification procedure of this product? Also, I would like to confirm the transformed E.coli were not included in the final protein sample. If the purification steps include sonication, 0.22 um filtration and affinity chromatography purification, there would be helpful information for us to conclude the E.coli were excluded from the protein sample.

The purification process of recombinant protein production includes sonication, 0.22 um filtration and affinity chromatography purification.