Recombinant Human CDK2AP1, His-tagged

• Cat.No.: CDK2AP1-1936H
• Product Overview: Recombinant CDK2AP1 protein fused to his-tag was expressed inE.coliand purified by using conventional chromatography techniques. 16.6kDa (152aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: CDK2AP1 is a specific CDK2-associated protein, which is thought to negatively regulate CDK2 activity by sequestering monomeric CDK2, and targeting CDK2 for proteolysis. Also, interact with DNA polymerase alpha/primase and mediate the phosphorylation of the large p180 subunit, which suggested the regulatory role in DNA replication during S phase of the cell cycle. A similar gene in hamster was isolated from, and functions as a growth suppressor of normal keratinocytes.
• Purity: > 95% by SDS – PAGE.
• Physical Appearance: Liquid.
• Formulation: 20mM Tris-HCl buffer (pH8.0) containing 50% glycerol, 2mM DTT.
• Concentration: 0.25 mg/ml (determined by Bradford assay).
• Sequences Of Amino Acids: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMSY KPNLAA H MPA AALNAAGSVH SPSTSMATSS QYRQLLSDYG PPSLGYT QGTGNS QV P Q S KY AELLAIIEEL GKEIRPTYAG SKSAMERLKR GIIHARGLVR ECLAET ERNA R S.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: CDK2AP1 cyclin-dependent kinase 2 associated protein 1 [ Homo sapiens ]
• Synonyms: CDK2AP1; cyclin-dependent kinase 2 associated protein 1; DOC1; ST19; DORC1; doc-1; p12DOC-1; CDK2-associated protein 1; Deleted in oral cancer-1; deleted in oral cancer 1; putative oral cancer suppressor; cyclin-dependent kinase 2-associated protein 1; CDKAP1
• Gene ID: 8099
• mRNA Refseq: NM_004642
• Protein Refseq: NP_004633
• MIM: 602198
• UniProt ID: O14519
• Chromosome Location: 12q24.31
• Function: DNA binding; protein binding