"CDK2/CCNE2" Related Products

Recombinant Human CDK2/CCNE2 Active protein, GST-tagged

Cat.No.: CDK2-CCNE2-311H
Product Overview: Recombinant full-length human CDK2 and CCNE2 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins.
Description: CDK2 is a member of the Cyclin-Dependent Kinase family that is ubiquitously expressed. CDK2 is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. CDK2 associates with and is regulated by the regulatory subunits of the complex including Cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). CDK2 phosphorylates multiple cellular substrates including SMAD3 and FOXO1. Phosphorylation of FOXO1 leads to its inhibition.
Source: Insect cell
Species: Human
Tag : GST
Form: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol
Bio-activity: The specific activity of CDK2/CyclinE2 was determined to be 60nmol/min/mg as per activity protocol.
Molecular Mass: CDK2 ~58 kDa and CCNE2 ~72 kDa
Purity: The purity of CDK2/CCNE2 was determined to be >90% by densitometry.
Applications: Kinase Assay; WB
Stability: 1 year at -70 centigrade
Storage: Store product at -70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Concentration: 0.1 μg/μL
Gene Name: cyclin dependent kinase 2 [ Homo sapiens (human) ]; cyclin E2 [ Homo sapiens (human) ]
Official Symbol: CDK2; CCNE2
Synonyms: CDKN2; p33; CDK2; CCNE2; CyclinE2
Gene ID: 1017; 9134
mRNA Refseq: NM_001798; NM_057749
Protein Refseq: NP_001789; NP_001228
MIM: 116953; 603775
UniProt ID: P24941; P24864

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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