Recombinant Human CDK3/CCNE2 Active protein, GST-tagged CDK3-CCNE2-312H

Cat.No.:	CDK3-CCNE2-312H
Product Overview:	Recombinant full-length human CDK3 and CCNE2 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins.
Description:	CDK3/CyclinE is a member of the cyclin-dependent protein kinase family and promotes entry into S phase, in part by activating members of the E2F family of transcription factors. CDK3 can also associate with cyclin C and phosphorylates the retinoblastoma 1 protein to promote exit from G0. The CDK3 gene has been mapped to chromosomal location of 17q22-qter, telomeric to the BRCA1 gene by somatic cell hybrids analysis. The presence of a single point mutation in the CDK3 gene from several Mus musculus strains commonly used in the laboratory has been reported.
Source:	Insect cell
Species:	Human
Tag :	GST
Form:	50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol
Bio-activity:	The specific activity of CDK3/CyclinE2 was determined to be 80nmol/min/mg as per activity protocol.
Molecular Mass:	CDK3 ~60 kDa and CCNE2 ~72 kDa
Purity:	The purity of CDK3/CCNE2 was determined to be >95% by densitometry.
Applications:	Kinase Assay; WB
Stability:	1 year at -70 centigrade
Storage:	Store product at -70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Concentration:	0.1 μg/μL

Download Datasheet

Gene Name:	cyclin dependent kinase 3 [ Homo sapiens (human) ]; cyclin E2 [ Homo sapiens (human) ]
Official Symbol:	CDK3; CCNE2
Synonyms:	CDK3; CCNE2; CYCE2
Gene ID:	1018; 9134
mRNA Refseq:	NM_001258; NM_057749
Protein Refseq:	NP_001249; NP_001228
MIM:	123828; 603775
UniProt ID:	Q00526; P24864

Online Inquiry