"CDK3/CCNE2" Related Products

Recombinant Human CDK3/CCNE2 Active protein, GST-tagged

Cat.No.: CDK3-CCNE2-312H
Product Overview: Recombinant full-length human CDK3 and CCNE2 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins.
Description: CDK3/CyclinE is a member of the cyclin-dependent protein kinase family and promotes entry into S phase, in part by activating members of the E2F family of transcription factors. CDK3 can also associate with cyclin C and phosphorylates the retinoblastoma 1 protein to promote exit from G0. The CDK3 gene has been mapped to chromosomal location of 17q22-qter, telomeric to the BRCA1 gene by somatic cell hybrids analysis. The presence of a single point mutation in the CDK3 gene from several Mus musculus strains commonly used in the laboratory has been reported.
Source: Insect cell
Species: Human
Tag : GST
Form: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol
Bio-activity: The specific activity of CDK3/CyclinE2 was determined to be 80nmol/min/mg as per activity protocol.
Molecular Mass: CDK3 ~60 kDa and CCNE2 ~72 kDa
Purity: The purity of CDK3/CCNE2 was determined to be >95% by densitometry.
Applications: Kinase Assay; WB
Stability: 1 year at -70 centigrade
Storage: Store product at -70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Concentration: 0.1 μg/μL
Gene Name: cyclin dependent kinase 3 [ Homo sapiens (human) ]; cyclin E2 [ Homo sapiens (human) ]
Official Symbol: CDK3; CCNE2
Synonyms: CDK3; CCNE2; CYCE2
Gene ID: 1018; 9134
mRNA Refseq: NM_001258; NM_057749
Protein Refseq: NP_001249; NP_001228
MIM: 123828; 603775
UniProt ID: Q00526; P24864

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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