| Species : |
Human |
| Source : |
Insect Cells |
| Tag : |
GST |
| Description : |
CDK3/CyclinE is a member of the cyclin-dependent protein kinase family and promotes entry into S phase, in part by activating members of the E2F family of transcription factors. CDK3 can also associate with cyclin C and phosphorylates the retinoblastoma 1 protein to promote exit from G0. The CDK3 gene has been mapped to chromosomal location of 17q22-qter, telomeric to the BRCA1 gene by somatic cell hybrids analysis. The presence of a single point mutation in the CDK3 gene from several Mus musculus strains commonly used in the laboratory has been reported. |
| Form : |
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol |
| Bio-activity : |
The specific activity of CDK3/CyclinE2 was determined to be 80nmol/min/mg as per activity protocol. |
| Molecular Mass : |
CDK3 ~60 kDa and CCNE2 ~72 kDa |
| Purity : |
The purity of CDK3/CCNE2 was determined to be >95% by densitometry. |
| Applications : |
Kinase Assay; WB |
| Stability : |
1 year at -70 centigrade |
| Storage : |
Store product at -70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. |
| Concentration : |
0.1 μg/μL |
