Recombinant Human Cell Division Cycle Associated 8, His-tagged

• Cat.No.: CDCA8-3526H
• Product Overview: Recombinant human CDCA8 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques (19-280aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: CDCA8, also known as Borealin, is a component of the chromosomal passenger complex (CPC). The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly.In the complex, CDCA8 protein may be required to direct the CPC to centromeric DNA. Deletion of CDCA8 delays mitotic progression and causes kinetochore-spindle to mis-attach.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT,50% glycerol, 1mM EDTA, 0.2M NaCl
• Molecular Weight: 31.6 kDa (283aa)confirmed by MALDI-TOF
• Purity: > 90% by SDS - PAGE
• Concentration: 0.25 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MRKLASFLKD FDREVEIRIK QIESDRQNLL KEVDNLYNIE ILRLPKALRE MNWLDYFALG GNKQALEEAATADLDITEIN KLTAEAIQTP LKSAKTRKVI QVDEMIVEEE EEEENERKNL QTARVKRCPP SKKRTQSIQG KGKGKRSSRA NTVTPAVGRLEVSMVKPTPG LTPRFDSRVF KTPGLRTPAA GERIYNISGN GSPLADSKEI FLTVPVGGGE SLRLLASDLQ RHSIAQLDPE ALGNIKKLSNRLAQICSSIR THK
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C.Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: CDCA8 cell division cycle associated 8 [ Homo sapiens ]
• Synonyms: CDCA8; BOR; DasraB; MESRGP; BOREALIN; FLJ10468; FLJ12042; borealin; Dasra B; dasra-B; hDasra-B; OTTHUMP00000004514; cell division cycle-associated protein 8; pluripotent embryonic stem cell-related gene 3 protein
• Gene ID: 55143
• mRNA Refseq: NM_018101
• Protein Refseq: NP_060571
• MIM: 609977
• UniProt ID: Q53HL2
• Chromosome Location: 1p34.3
• Function: protein binding

Citation

Efficacy of newly discovered DNA aptamers targeting AXL in a lung cancer cell with acquired resistance to Erlotinib

Journal: Translational Cancer Research    PubMed ID: 35116429    Data: 2021/2/1

Authors: Ji An Hwang, Jae Young Hur, Chang-Min Choi

Article Snippet:The resulting aptamers were used in the next SELEX rounds.The resulting aptamers were used in the next SELEX rounds.. The target protein used for aptamers selection were recombinant Human AXL extracellular domain (Met 1-Pro 449), fused with a polyhistidine tag at the C-terminus produced in Human Cell (Creative BioMart, Shirley, NY, USA).