Recombinant Human CENPT Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: CENPT-5871H
• Product Overview: CENPT MS Standard C13 and N15-labeled recombinant protein (NP_079358) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by the presence of long arrays of nucleosomes in which CENPA replaces histone H3. CENPT is an additional factor required for centromere assembly.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 60.4 kDa
• AA Sequence: MADHNPDSDSTPRTLLRRVLDTADP RTPRRPRSARAGARRALLETASPRK LSGQTRTIARGRSHGARSVGRSAHI QASGHLEEQTPRTLLKNILLTAPES SILMPESVVKPVPAPQAVQPSRQES SCGSLELQLPELEPPTTLAPGLLAP GRRKQRLRLSVFQQGVDQGLSLSQE PQGNADASSLTRSLNLTFATPLQPQ SVQRPGLARRPPARRAVDVGAFLRD LRDTSLAPPNIVLEDTQPFSQPMVG SPNVYHSLPCTPHTGAEDAEQAAGR KTQSSGPGLQKNSPGKPAQFLAGEA EEVNAFALGFLSTSSGVSGEDEVEP LHDGVEEAEKKMEEEGVSVSEMEAT GAQGPSRVEEAEGHTEVTEAEGSQG TAEADGPGASSGDEDASGRAASPES ASSTPESLQARRHHQFLEPAPAPGA AVLSSEPAEPLLVRHPPRPRTTGPR PRQDPHKAGLSHYVKLFSFYAKMPM ERKALEMVEKCLDKYFQHLCDDLEV FAAHAGRKTVKPEDLELLMRRQGLV TDQVSLHVLVERHLPLEYRQLLIPC AYSGNSVFPAQSGPTRTRPLEQKLI SEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: CENPT centromere protein T [ Homo sapiens (human) ]
• Official Symbol: CENPT
• Synonyms: CENPT; centromere protein T; C16orf56, chromosome 16 open reading frame 56; CENP T; FLJ13111; interphase centromere complex protein 22; CENP-T; C16orf56; FLJ43376
• Gene ID: 80152
• mRNA Refseq: NM_025082
• Protein Refseq: NP_079358
• MIM: 611510
• UniProt ID: Q96BT3