Recombinant Human CFI protein, His-tagged

• Cat.No.: CFI-290H
• Product Overview: Recombinant Human CFI(Ile340~Val583) fused with His tag at N-terminal was expressed in E. coli.

Specification

• Description: This gene encodes a serine proteinase that is essential for regulating the complement cascade. The encoded preproprotein is cleaved to produce both heavy and light chains, which are linked by disulfide bonds to form a heterodimeric glycoprotein. This heterodimer can cleave and inactivate the complement components C4b and C3b, and it prevents the assembly of the C3 and C5 convertase enzymes. Defects in this gene cause complement factor I deficiency, an autosomal recessive disease associated with a susceptibility to pyogenic infections. Mutations in this gene have been associated with a predisposition to atypical hemolytic uremic syndrome, a disease characterized by acute renal failure, microangiopathic hemolytic anemia and thrombocytopenia. Primary glomerulonephritis with immune deposits and age-related macular degeneration are other conditions associated with mutations of this gene.
• Source: E. coli
• Species: Human
• Tag: His
• Form: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300
• Molecular Mass: 29.1kDa
• Protein length: Ile340~Val583
• Endotoxin: Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
• Purity: > 95%
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Reconstitution: Reconstitute in PBS or others.

Gene Information

• Gene Name: CFI complement factor I [ Homo sapiens ]
• Official Symbol: CFI
• Synonyms: CFI; complement factor I; I factor (complement) , IF; C3b INA; C3b inactivator; FI; KAF; Konglutinogen activating factor; C3b-inactivator; C3B/C4B inactivator; complement component I; light chain of factor I; Konglutinogen-activating factor; complement factor I heavy chain; complement control protein factor I; IF; AHUS3; C3BINA; C3b-INA
• Gene ID: 3426
• mRNA Refseq: NM_000204
• Protein Refseq: NP_000195
• MIM: 217030
• UniProt ID: P05156
• Chromosome Location: 4q25
• Pathway: Complement and coagulation cascades, organism-specific biosystem; Complement and coagulation cascades, conserved biosystem; Complement cascade, organism-specific biosystem; Immune System, organism-specific biosystem; Innate Immune System, organism-specific biosystem; Regulation of Complement cascade, organism-specific biosystem; Staphylococcus aureus infection, organism-specific biosystem
• Function: peptidase activity; scavenger receptor activity; serine-type endopeptidase activity