Recombinant Human CFTR Protein (Pro1181-End), N-His-tagged

• Cat.No.: CFTR-12H
• Product Overview: Purified recombinant protein of Human cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7) (CFTR), Pro1181-End, with N-terminal His tag, expressed in E. coli.

Specification

• Description: This gene encodes a member of the ATP-binding cassette (ABC) transporter superfamily. The encoded protein functions as a chloride channel, making it unique among members of this protein family, and controls ion and water secretion and absorption in epithelial tissues. Channel activation is mediated by cycles of regulatory domain phosphorylation, ATP-binding by the nucleotide-binding domains, and ATP hydrolysis. Mutations in this gene cause cystic fibrosis, the most common lethal genetic disorder in populations of Northern European descent. The most frequently occurring mutation in cystic fibrosis, DeltaF508, results in impaired folding and trafficking of the encoded protein. Multiple pseudogenes have been identified in the human genome.
• Source: E. coli
• Species: Human
• Tag: N-His
• Molecular Mass: 33.9 kDa
• Protein Length: Pro1181-End
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 50mM Tris, 8M Urea, pH8.0.

Gene Information

• Gene Name: CFTR CF transmembrane conductance regulator [ Homo sapiens (human) ]
• Official Symbol: CFTR
• Synonyms: CFTR; CF transmembrane conductance regulator; CF; MRP7; ABC35; ABCC7; CFTR/MRP; TNR-CFTR; dJ760C5.1; cystic fibrosis transmembrane conductance regulator; cAMP-dependent chloride channel; channel conductance-controlling ATPase; cystic fibrosis transmembrane conductance regulating; cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7); EC 5.6.1.6
• Gene ID: 1080
• mRNA Refseq: NM_000492
• Protein Refseq: NP_000483
• MIM: 602421
• UniProt ID: P13569