Recombinant Human CILP protein, T7/His-tagged

• Cat.No.: CILP-69H
• Product Overview: Recombinant human CILP - C1 cDNA (22 - 724aa, derived from BC035776) fused with T7-His-TEV cleavage site Tag (29aa) at N-terminal was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: T7/His
• Form: 0.1 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, arginine, DTT and Glycerol.
• AA Sequence: MASMTGGQQMGRGHHHHHHGNLYFQ GGEFRQTMLTQSVRRVQPGKKNPSI FAKPADTLESPGEWTTWFNIDYPGG KGDYERLDAIRFYYGDRVCARPLRL EARTTDWTPAGSTGQVVHGSPREGF WCL NREQRPGQNCSNYTVRFLCPPGSLR RDTERIWSPWSPWSKCSAACGQTGV QTRTRICLAEMVSLCSEASEEGQHC MGQDCTACDLTCPMGQVNADCDACM CQDFMLHGAVSLPGGAPASGAAIYL LTKTPKLLTQTDSDGRFRIPGLCPD GKSILKITKVKFAPIVLTMPKTSLK AATIKAEFVRAETPYMVMNPETKAR RAGQSVSLCCKATGKPRPDKYFWYH NDTLLDPSLYKHESKLVLRKLQQHQ AGEYFCKAQSDAGAVKSKVAQLIVI ASDETPCNPVPESYLIRLPHDCFQN ATNSFYYDVGRCPVKTCAGQQDNGI RCRDAVQNCCGISKTEEREIQCSGY TLPTKVAKECSCQRCTETRSIVRGR VSAADNGEPMRFGHVYMGNSRVSMT GYKGTFTLHVPQDTERLVLTFVDRL QKFVNTTKVLPFNKKGSAVFHEIKM LRRKKPITLEAMETNIIPLGEVVGE DPMAELEIPSRSFYRQNGEPYIGKV KASVTFLDPRNISTATAAQTDLNFI NDEGDTFPLRTYGMFSVDFRDEVTS EPLNAGKVKVHLDSTQVKMPEHIST VKLWSLNPDTGLWEEEGDFKFENQR RNKR
• Purity: >90% by SDS-PAGE
• Applications: 1. May be used for in vitro CILP N-terminal protein C1 mediated IGF-1 antagonist activities for osteoblast cell differentiatio regulation study with this protein as either coating matrix protein or as soluble factor.2. May be used for CILP C1 protein – protein interaction assay.3. May be used as enzymatic substrate for various proteases.4. May be used for specific antibody production.
• Storage: Keep at -80centigrade for long term storage. Product is stable at 4 centigrade for at least 30 days.

Gene Information

• Gene Name: CILP cartilage intermediate layer protein, nucleotide pyrophosphohydrolase [ Homo sapiens ]
• Official Symbol: CILP
• Synonyms: CILP; cartilage intermediate layer protein, nucleotide pyrophosphohydrolase; cartilage intermediate layer protein 1; HsT18872; cartilage intermediate-layer protein; cartilage intermediate layer protein 1 C1; cartilage intermediate layer protein 1 C2; CILP
• Gene ID: 8483
• mRNA Refseq: NM_003613
• Protein Refseq: NP_003604
• MIM: 603489
• UniProt ID: O75339
• Chromosome Location: 15q22
• Function: NOT alkaline phosphatase activity; NOT nucleotide diphosphatase activity