Recombinant Human CLGN protein, His & T7-tagged

• Cat.No.: CLGN-1660H
• Product Overview: Recombinant Human CLGN aa. (Asp23~Glu293 (Accession # O14967)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His&T7
• Protein Length: Asp23~Glu293
• Description: Calmegin is a testis-specific endoplasmic reticulum chaperone protein. CLGN may play a role in spermatogeneisis and infertility.
• Form: Freeze-dried powder
• Molecular Mass: 42kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >90%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in sterile ddH2O.
• Isoelectric Point: 4.6

Gene Information

• Gene Name: CLGN calmegin [ Homo sapiens (human) ]
• Official Symbol: CLGN
• Synonyms: CLGN; calmegin; testis tissue sperm-binding protein Li 79P
• Gene ID: 1047
• mRNA Refseq: NM_001130675.1
• Protein Refseq: NP_001124147.1
• UniProt ID: O14967