| Description : |
Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. May be involved in metabolic regulation; may be involved in recruitment of the CCR4-NOT complex to deadenylation target mRNAs involved in energy metabolism. Involved in mitotic progression and regulation of the spindle assembly checkpoint by regulating the stability of MAD1L1 mRNA. Can repress transcription and may link the CCR4-NOT complex to transcriptional regulation; the repressive function may involve histone deacetylases. Involved in the maintenance of embryonic stem (ES) cell identity. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
81.9 kDa |
| AA Sequence : |
MADKRKLQGEIDRCLKKVSEGVEQF EDIWQKLHNAANANQKEKYEADLKK EIKKLQRLRDQIKTWVASNEIKDKR QLIDNRKLIETQMERFKVVERETKT KAYSKEGLGLAQKVDPAQKEKEEVG QWLTNTIDTLNMQVDQFESEVESLS VQTRKKKGDKDKQDRIEGLKRHIEK HRYHVRMLETILRMLDNDSILVDAI RKIKDDVEYYVDSSQDPDFEENEFL YDDLDLEDIPQALVATSPPSHSHME DEIFNQSSSTPTSTTSSSPIPPSPA NCTTENSEDDKKRGRSTDSEVSQSP AKNGSKPVHSNQHPQSPAVPPTYPS GPPPAASALSTTPGNNGVPAPAAPP SALGPKASPAPSHNSGTPAPYAQAV APPAPSGPSTTQPRPPSVQPSGGGG GGSGGGGSSSSSNSSAGGGAGKQNG ATSYSSVVADSPAEVALSSSGGNNA SSQALGPPSGPHNPPPSTSKEPSAA APTGAGGVAPGSGNNSGGPSLLVPL PVNPPSSPTPSFSDAKAAGALLNGP PQFSTAPEIKAPEPLSSLKSMAERA AISSGIEDPVPTLHLTERDIILSST SAPPASAQPPLQLSEVNIPLSLGVC PLGPVPLTKEQLYQQAMEEAAWHHM PHPSDSERIRQYLPRNPCPTPPYHH QMPPPHSDTVEFYQRLSTETLFFIF YYLEGTKAQYLAAKALKKQSWRFHT KYMMWFQRHEEPKTITDEFEQGTYI YFDYEKWGQRKKEGFTFEYRYLEDR DLQTRTRPLEQKLISEEDLAANDIL DYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
