Active Recombinant Human CNTN3 protein(Met1-Ser1002, 708Asp/Asn), His-tagged

• Cat.No.: CNTN3-672H
• Product Overview: Active Recombinant Human CNTN3 protein(NP_065923.1) (Met 1-Ser1002, 708 Asp/Asn) precursor was expressed in HEK293 with a C-terminal polyhistidine tag.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-1002 aa,708 Asp/Asn
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of C6 Rat brain glial cells. When 5 x 10^4 cells/well are added to CNTN3 coated plates (0.8 μg/ml and 100 μl/well), approximately 35%-55% will adhere specifically after 60 minutes at 37°C.
• Molecular Mass: The recombinant human CNTN3 consists of 994 amino acids and has a calculated molecular mass of 109.2 kDa. By SDS-PAGE under reducing conditions, the apparent molecular mass of rh CNTN3 is approximately 120-130 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CNTN3 contactin 3 (plasmacytoma associated) [ Homo sapiens ]
• Official Symbol: CNTN3
• Synonyms: CNTN3; contactin 3 (plasmacytoma associated); PANG; contactin-3; BIG 1; plasmacytoma-associated neuronal glycoprotein; brain-derived immunoglobulin superfamily protein 1; PCS; BIG-1; KIAA1496
• Gene ID: 5067
• mRNA Refseq: NM_020872
• Protein Refseq: NP_065923
• MIM: 601325
• UniProt ID: Q9P232